Anti dsDNA IgG (GWB-521215)


96 Wells


Introduction Anti-dsDNA IgG test is used for initial diagnosis of Systemic Lupus Erythematosus (SLE) and for diagnosis of SLE different diseases. Besides the determination of high titers of antinuclear antibody (ANA), the determination of autoantibodies against dsDNA is one of the ACR criteria (American College of Rheumatology) for the diagnosis of Systemic Lupus Erythematosus (SLE). The determination of the concentration of antibodies can be used to monitor treatment success and predict possible attacks of the disease (SLE).
Intended Use Anti dsDNA IgG kit is an indirect solid phase enzyme immunometric assay (ELISA) designed for the quantitative measurement of IgG class antibodies directed against dsDNA in human serum or plasma. Anti dsDNA IgG is intended for laboratory use only.
Principle of the Assay Anti-dsDNA IgG test is based on the binding of serum or plasma IgG antibodies on dsDNA coated on the microplates. The antibodies in calibrators, controls or prediluted patient samples bind into the inner surface of the wells. After a 30 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. An anti-human-IgG horseradish peroxidase conjugate solution recognizes the IgG class antibodies bound to the immobilized dsDNA antigens. After a 30 minutes incubation any excess of enzyme conjugate, which is not specifically bound, is washed away with wash buffer. A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solution color changes into yellow. The amount of color is directly proportional to the concentration of the anti-dsDNA IgG antibodies present in the original sample.

Additional Information

Name Anti dsDNA IgG (GWB-521215)
Related Product Names Anti Double Stranded DNA IgG ELISA, Anti Double Stranded DNA Immunoglobulin G ELISA, Anti dsDNA IgG ELISA, Anti dsDNA Immunoglobulin G ELISA, IgG Antibodies to dsDNA, IgG Antibodies against dsDNA, Rheumatology Assay
Molecular Weight 0.5
Concentration Lot Specific
Application Enzyme immunoassay for the quantitative determination of anti dsDNA in human serum or plasma
Reactivity Human
Datasheets/Manuals Printable manual for GWB-521215
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the dark.
Intended Use Research Use Only