Anti PR 3 (c-ANCA)

GWB-521205

96 Wells
$349.00

Description

1. INTRODUCTION
Anti-neutrophilic-cytoplasm antibodies (ANCA) represents a group of autoantibodies directed towards the cytoplasmatic components of the neutrophilic granulocytes and monocytes. The classical methods for the determination of ANCA are the immunofluorescent methods. With these indirect immunofluorescence techniques two main patterns are recognized, a cytoplasmatic (c-ANCA) and a perinuclear (p-ANCA) type. The main antigen for the c-ANCA is the proteinase 3 (PR3), which is a serine proteinase present in primary granules. Antibodies of p-ANCA positive sera are mainly directed to myeloperoxidase (MPO). Antibodies to other antigens e.g. lactoferrin, elastase, cathepsin-G and also lysozyme often result in a similar p-ANCA pattern. Beside different untypical variants of p-ANCA IF patterns granulocyte specific antinuclear antibodies (GS-ANA) is indistinguishable from p-ANCA. This makes a clear interpretation and classification of the IF patterns difficult. Therefore every positive IF-ANCA findings esp. p-ANCA should be differenciated by ELISA techniques using purified antigens. A survey of documented clinical indications of specific ANCA is given in the table below. PR3-ANCA and MPO-ANCA are reliable serologic markers in the diagnostics of vasculitides. PR3-ANCA is the classical autoantigen in Wegener´s granulomatosis with a clinical specificity of more than 95%. c-ANCA is documented to be present in different diseases. Anti-MPO antibodies are highly specific for idiopathic and vasculitis associated crescentic glomerulonephritis and also for classic polyarteritis nodosa, Churg-Strauss syndrome and the polyangitis overlap syndrome without renal involvement. With respect to sensitivity, either MPO or PR-3 antibodies were found in 77 to 100% of patients with idiopathic and vasculitis associated crescentic glomerulonephritis. In WG, anti-MPO antibodies were detected only occasionally and generally in patients negative for PR-3 antibodies. The MPO and PR-3 specific ELISA methods can provide an important confirmatory result for two of the more important of the identified antigens. ELISA is also useful for interpreting "difficult" samples by IFA such as those which exhibit several antibodies simultaneously or those with high background fluorescence.

2. INTENDED USE
Anti PR3 is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgG class autoantibodiesagainst proteinase 3 (PR3) in human serum or plasma. The assay is intended for laboratory research use only. Anti PR3 (c-ANCA) kit is intended for laboratory use only.

3. PRINCIPLE OF THE ASSAY
The anti-PR3 IgG test is based on the binding of present antibodies on the human neutrophil proteinase 3 coated into the microplates. Any present antibodies in calibrators, controls or pre-diluted patient samples bind to the inner surface of the wells. After a 60 minutesincubation the microplate is washed with wash buffer for removing non-reactive Serum or Plasma components. An anti-human-IgG horseradish peroxidase conjugate solution recognize IgG class antibodies bound to the immobilized antigens. After a 30 minutes incubation any excess enzyme conjugate, which is not specifically bound is washed away with wash buffer. A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solution color change into yellow. The amount of color is directly proportional to the concentration of IgG antibodies present in the original sample.

Additional Information

Name Anti PR 3 (c-ANCA)
Related Product Names Cytoplasmic antineutrophil cytoplasmic antibodies (c-ANCA) ELISA, Cytoplasmic antineutrophil cytoplasmic antibodies ELISA, c-ANCA ELISA, Anti PR3 ELISA, ANCA / Vasculitis Assay
Molecular Weight 0.5
Concentration Lot Specific
Applications Enzyme immunoassay for the quantitative determination of anti PR3 in human serum or plasma
Reactivity Human
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the dark.
Datasheets/Manuals Printable manual for GWB-521205
Intended Use Research Use Only