Anti SS-A (GWB-521216)

GWB-521216

96 Wells
$349.00

Description

1. INTRODUCTION
The SSA/Ro antigens are nuclear and cytoplasmic polypeptides which serve as autoantigens in systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). They contain two major isoforms of 60 and 52 kD. The former is the native antigen while the latter is a major autoantigen in its denatured form. The clinical relevance of anti SSA/Ro antibodies in rheumatic diseases has also been considered. Initially these antibodies were thought to be an epiphenomenon of autoimmune diseases. Recent studies have shown that they are associated with specific clinical manifestations and disease subsets. Furthermore, animal models have demonstrated that they may enhance tissue damage. It seems that anti-SSA/Ro antibodies may play a role in the pathogenicity of SLE and SS. Sjögren's syndrome is a chronic inflammatory autoimmune disease, characterized by destruction of exocrine glands (minor salivary glands, lacrimal glands, parotid) mediated by T lymphocytes. It has a frequency of 1% and it mainly affects women (9:1) from 50 years, it seems to be associated with a genetic predisposition related to HLA-DRw52 (which increases the risk of developing disease by a factor of 20) and HLA DR3 genes and probably to viral infection by EBV and HTLV-1. SS also produces an excessive activation of B cells with production of autoantibodies such as rheumatoid factor (RF), anti-SS-A/Ro, anti-SS-B/La. In patients with SS they present in up to 60% of patients when tested by immunodiffusion and up to 90% when tested by ELISA, and are associated with more frequent extraglandular involvement. They may also be present in approximately 30% of patients with SLE. Patients presenting high titers in ELISA, especially for anti-Ro 52, are a risk factor for neonatal lupus.

2. INTENDED USE
Anti-SS-A kit is an enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of IgG class antibodies directed against the SS-A/Ro antigen in human serum or plasma. Anti SS-A kit is intended for laboratory use only.

3. PRINCIPLE OF THE ASSAY
The anti-SS-A test is based on the binding of antibodies present in calibrators, controls or pre-diluted patient samples to the SS-Aantigen coated into the inner surface of the microplate wells. After a 30 minute incubation the microplate is washed with wash buffer to remove the non-reactive serum components. An anti-human-IgG horseradish peroxidase conjugate solution recognizes IgG class antibodies bound to the immobilized antigens. After a 30 minute incubation excess enzyme conjugate, which is not specifically bound is washed away with wash buffer. A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation color development is stopped by adding the stop solution. The solution turns yellow at this point. The level of color is directly proportional to the concentration of IgG antibodies present in the original sample.

Additional Information

Name Anti SS-A (GWB-521216)
Related Product Names Anti E3 ubiquitin-protein ligase TRIM21 ELISA, Anti 52 kDa Ro protein ELISA Kit, Anti 52 kDa ribonucleoprotein autoantigen Ro / SS-A ELISA Kit, Anti RING finger protein 81 ELISA, Anti- Ro(SS-A) ELISA Kit, Anti Sjoegren syndrome type A antigen ELISA Kit, A
Molecular Weight 0.5
Concentration Lot Specific
Application Enzyme immunoassay for the quantitative determination of anti SS-A in human serum or plasma
Reactivity Human
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the dark.
Datasheets/Manuals Printable manual for GWB-521216
Intended Use Research Use Only