Anti SS-B (GWB-521217)

GWB-521217

96 Wells
$349.00

Description

1. INTRODUCTION
SS-B/La, an ubiquitous nuclear protein of 46-48 kD, is a target antigen of autoantibodies in SLE and Sjogren's syndrome and is involved in the maturation of RNA polymerase III transcripts. SS-B/La shuttles between nucleus and cytoplasm and exists both free and as a component of the SSA/SSB ribonucleoprotein cytoplasmic particle. Autoantibodies to SSB are detected by ELISA in 25% of SLE, in 80% of subacute cutaneous lupus, in the majority of infants with complete heart block and in 70-90% of primary and 50% of secondary Sjögren’s syndrome. Sjögren's syndrome is a chronic inflammatory autoimmune desease, characterized by destruction of exocrine glands (minor salivary glands, lacrimal glands, parotid) mediated by T lymphocytes. It has a frequency of 1%, mainly affects women (9:1) over the age of 50 years, and seems to be associated with a genetic predisposition related to HLA-DRw52 (which increases the risk of developing disease by a factor of 20) and HLA DR3 genes and probably to viral infection by EBV and HTLV-1. SS also produces an excessive activation of B cells with production of autoantibodies such as rheumatoid factor (RF), anti-SS-B/Ro, anti-SS-B/La. In patients with SS these antibodies present in 60% of cases when tested by immunodiffusion and up to 90% when tested by ELISA, and they are associated with more frequent extraglandular involvement. They may also be present in approximately 30% of patients with SLE. High titers in ELISA, especially for anti-Ro 52, are a risk factor for neonatal lupus.

2. INTENDED USE
Anti-SS-B kit is an indirect enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of IgG class antibodies directed against the SS-B/Ro antigen in human serum or plasma. Anti SS-B kit is intended for laboratory use only.

3. PRINCIPLE OF THE ASSAY
Anti-SS-B is based on the binding of antibodies present in calibrators, controls or pre-diluted patient samples to the SS-B antigen coated into the inner surface of the microplate wells. After a 30 minutes incubation the microplate is washed with wash buffer to remove the non-reactive serum components. An anti-human-IgG horseradish peroxidase conjugate solution recognizes IgG class antibodies bound to the immobilized antigens. After a 30 minutes incubation excess enzyme conjugate, which is not specifically bound is washed away with wash buffer.A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation color development is stopped by adding the stop solution. The solution turns yellow at this point. The level of color is directly proportional to the concentration of IgG antibodies present in the original sample.

Additional Information

Name Anti SS-B (GWB-521217)
Related Product Names Anti - Lupus La protein ELISA Kit, Anti La Autoantigen ELISA, Anti La ribonucleoprotein ELISA, Anti Sjoegren syndrome type B antigen ELISA Kit, Antibodies to SSB ELISA, Antibodies agaisnt SSB ELISA Kit, Anti SSB ELISA, Rheumatology Assay
Molecular Weight 0.5
Concentration Lot Specific
Application Enzyme immunoassay for the quantitative determination of anti SS-B in human serum or plasma
Reactivity Human
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the dark.
Datasheets/Manuals Printable manual for GWB-521217
Intended Use Research Use Only