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Thyroid peroxidase (TPO) present at the apical plasma membrane of human thyroid cells catalyzes the first step in thyroid hormone synthesis. TPO oxidizes iodide-anions and iodinates some tyrosyl residues of thyreoglobulin (TG), leading to the formation of monoiodotyrosine (MIT) and diiodotyrosine (DIT). Coupling of MIT and DIT yields triiodotyronine (T3) and tetra-iodotyronine (T4). TPO (previously known as thyroid microsomal antigen) is a frequent epitope of autoantibodies in autoimmune thyroid disease. TPO autoantibodies (TPOAb) are detectable in over 90 % of patients with Hashimoto's thyroiditis and over 70 % of those with Graves' disease (autoimmune hyperthyroidism), irrespective of the functional state of the thyroid gland. Postpartum thyroiditis is a special form of autoimmune thyroiditis observed following pregnancy and may involve temporary hyperthyroidism or hypothyroidism. It affects about 5 % of all women within a year after giving birth. The risks are higher for women with autoimmune disorders (such as type 1 diabetes), positive anti-thyroid antibodies or a history of previous thyroid dysfunction. Moderately elevated TPOAb values are sometimes found in patients without other evidence of thyroid disease, especially in the elderly. The clinical significance of low levels of TPOAb in euthyroid subjects is still unknown. Whether these individuals should be considered normal remains in question until long-term follow-up studies show that they do not have an increased risk for developing thyroid dysfunction.

Thyroid peroxidase (TPO)

 Hashimoto's thyroiditis Graves' disease (= Morbus Basedow)

 initially hyperthyroidism: nervousness, weight loss, insomnia, tachycardia, heat intolerance etc. later gradual transition to chronic hypothyroidism: fatigue, decreased concentration, weight gain, constipation, cold intolerance etc. hyperthyroidism: exophthalmos, struma, tachycardia (Merseburg triad)

The presence of autoantibodies to TPO may be detected by indirect immunofluorescence and several quantitative immunoassays, e. g. radioimmunoassays and Enzyme-linked Immunosorbent Assay (ELISA).

The GenWay anti-TPO ELISA is intended for the quantitative determination of autoantibodies to TPO (thyroid peroxidase) in human serum or plasma (citrate).

The quantitative immunoenzymatic determination of autoantibodies to TPO is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strips are precoated with recombinant thyroid peroxidase (TPO) to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured TPO-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of TPO specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

Additional Information

Name Anti-TPO
Related Product Names Anti- Thyroid peroxidase ELISA Kit, Antibodies against Thyroid peroxidase ELISA, Antibodies against TPO ELISA Kit, Anti-PERT ELISA
Molecular Weight 0.5
Datasheets / Downloads GWB-521202 Datasheet
Concentration Lot Specific
Applications Enzyme immunoassay for the quantitative determination of autoantibodies to TPO (thyroid peroxidase) in human serum or plasma
Reactivity Human
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.
Intended Use Research Use Only