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Human BD-1 ELISA Kit

GWB-SKR002

1x96 well plate
$390.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human BD-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human BD-1 proteins within the range of 4-1000 pg/ml. The Human BD-1 ELISA is capable of recognizing both recombinant and naturally produced Human BD-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity. • Human BD-1 (47 aa) The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human BD-2, BD-3, BD-4

Additional Information

Name Human BD-1 ELISA Kit
Related Product Names Beta-defensin 1, BD-1, Defensin beta 1, hBD-1, HBD1, HBP1, DEFB1, HBD-1, HBP-1, DEFB101, DEFB-1, MGC51822
Drywet Surcharge 0
Gene Symbol DEFB1
Gene Family DEFB
Gene Fullname defensin beta 1
Gene id 1672
Alias Symbols BD1, BD-1, Beta-defensin 1, DEFB-1, DEFB101, Defensin, beta 1, HBD1, hBD-1, HBD-1, MGC51822
Protein Name Beta-defensin 1
Description of Target Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithel
Swissprot ID P60022
Protein Accession Num NP_005209.1
Nucleotide Accession Num NM_005218.3
Species Reactivity Human
Assay Info Quantitative Colorimentric Sandwich ELISA
Sensitivity 8 pg/mL
Kit Range 8-1,000 pg/mL
Standard Range 4-1000 pg/ml
Sample Type Cell lysates, sera and plasma
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Datasheets / Downloads GWB-SKR002 Datasheet
Specimen/Volumes Refer to Manual
Swiss Prot Number P60022
Specificity The Human BD-1 ELISA is capable of recognizing both recombinant and naturally produced Human BD-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Human: BD-1 (47 aa)
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: BD-2, BD-3, BD-4
Applications ELISA
GI Number 1672
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Kit Principle The Aviva Human BD-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human BD-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human BD-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Protein StandardLyophilized
Biotinylated Detection AntibodyLyophilized
400x Streptavidin-HRP30 μl
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Storage 4°C/6 Months
Intended Use Research Use Only