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Human BD-4 ELISA Kit

GWB-SKR005

1x96 well plate
$480.00
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Description

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Human BD-4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human BD-4 proteins within the range of 8-1000 pg/ml. The Human BD-4 ELISA is capable of recognizing both recombinant and naturally produced Human BD-4 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human BD-1 (36 aa), BD-1 (47 aa), BD-2, BD-3

Additional Information

Name Human BD-4 ELISA Kit
Related Product Names HBD-4, DEFB-4, HBD4, DEFB104B, Beta-defensin 4, BD-4
Kit Range 8-1,000 pg/mL
Sensitivity 8 pg/mL
Sample Type Cell lysates, sera and plasma
Kit Detection Colorimetric 450 nm
Gene Symbol DEFB104A
Gene Fullname defensin beta 104A
Gene id 140596
Alias Symbols BD-4, DEFB104, DEFB104B, DEFB4, DEFB-4, hBD-4, MGC118942, MGC118944, MGC118945
Swissprot ID Q8WTQ1
Protein Name Beta-defensin 104
Description of Target Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensi
Kit Principle The Aviva Human BD-4 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human BD-4 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human BD-4 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Protein StandardLyophilized (83 ng)
Biotinylated Detection AntibodyLyophilized
400x Streptavidin-HRP30 μl
Wash Buffer (15x)50 mL
Assay Diluent50 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Protein Accession Num NP_001035792.1
Nucleotide Accession Num NM_001040702.1
Assay Info Quantitative Colorimentric Sandwich ELISA
Standard Range 8-1000 pg/ml
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Specimen/Volumes Refer to Manual
Specificity The Human BD-4 ELISA is capable of recognizing both recombinant and naturally produced Human BD-4 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: BD-1 (36 aa), BD-1 (47 aa), BD-2, BD-3
Applications ELISA
GI Number 140596/503618
Format ELISA Kit. Refer to manual for specific component formats
Stability 6 months
Reconstitution and Storage 4C/6 Months
Reactivity Human
Kit Size 12 x 8-Well Microstrips
Storage 4°C/6 Months
Datasheets/Manuals Printable datasheet for GWB-SKR005
Intended Use Research Use Only