Serotonin ELISA Kit (GWB-D1BB69)



1x96 well plate
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"Serotonin is an intermediate product of tryptophan metabolism and is located primarily in the enterochromaffin cells of intestine, serotonergic neurons of the brain, platelets of the blood and is well established as a neurotransmitter in the central nervous system. Nearly all of the serotonin in circulating blood is concentrated in platelets. Altered concentrations of circulating serotonin have been implicated in several pathological conditions including chronic tension headache, schizophrenia, hypertension, Huntington´s disease, Duchenne´s muscular dystrophy and early acute appendicitis. The determination of serum serotonin levels is of high clinical significance for diagnostic assessment of carcinoid syndrome. An increasing interest in the determination of serotonin in platelets including uptake and release kinetics is expected in the near future. The sample preparation (derivatization of serotonin to N-acylserotonin) is part of the sample dilution and is achieved by incubation of the respective sample with the Acylation Reagent. The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards."

Additional Information

Name Serotonin ELISA Kit (GWB-D1BB69)
Availability In Stock
Related Product Names Serotonin ELISA Kit; 5-HT-5A; 5-HT-5; Serotonin receptor 5A Serotonin HTR5A, Serotonin
Sample Type Serum, Plasma, Platelets, Urine, Tissue Homogenate, Cell Culture Supernatant.
Swissprot ID P47898
NCBI Acc Number NP_076917.1
Standard Range 0.08 - 19.8 ng/mL.
Molecular Weight 40255
Specimen/Volumes 10 ul serum, platelets, urine, tissue homogenate et al., 50 uL plasma.
Substrate/Isotope PNPP 405 nm.
Concentration Lot Specific
Application ELISA
Reactivity Human
Kit Size 96 well
Storage The kit is shipped at ambient temperature and should be stored at 2C-8C. Keep away from heat or direct sun light. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2C-8&de
Datasheets/Manuals Printable datasheet for GWB-D1BB69
Intended Use Research Use Only