Human E-cadherin EIA Kit (GWB-40B393)





Product Name: Human E-cadherin EIA Kit

# of Samples: 1X96 Assays

Intended Use: The human E-cadherin E IA Kit is an in vitro enzyme immunoassa y ( EIA ) k i t for quantitative determination of soluble human E-cadherin in serum, urine, cell culture supernatants, and other biological fluids. This kit is suitable for quantitation of soluble human E-cadherin
This kit is for research use only. It is not for use in diagnostic or therapeutic procedures

Introduction: E-cadherin, also known as uvomorulin or Cell-CAM120/80, is one of the subclasses of cadherins, Ca2+-dependent cell adhesion molecules(1,2), found in epithelial cells in a variety of embryonic and adult tissues (3,4,5). Previous studies suggested that unstable or reduced expression of E-cadherin seems to be a common event in cancer progression, such as in lung carcinoma(6), gastric tumours(7), hepatocellular carcinoma(8), breast carcinoma(9,17), and prostatic tumours(10,11). Soluble forms of E-cadherin, 80~84 kDa protein, firstly found in the serum-free culture medium released from MCF-7 human carcinoma cells or artificially generated by trypsinising cells in the presence of calcium (3,4) and is presumed to be a degradation product of the 120 kDa form of intact E-cadherin generated by a Ca2+-dependent proteolytic action(1,12). Recently, the 80 kDa form of soluble E-cadherin was found to be circulating in biological fluids of healthy persons(14), and was shown that the serum levels were elevated in the tumour patients(13,15) and those with systemic inflammatory response syndrome and multiorgan dysfunction syndrome(16).

Principle of the test: The human E-cadherjn EIA Kit is a solid phase EIA based on a sandwich method that utilizes two mouse monoclonal anti-human E-cadherin antibodies to detect soluble E-cadherin by two-step procedure. One of the mouse monoclonal anti-human E-cadherin is bound to a microtiter plate to create the solid phase. Nonspecific binding is blocked by a blocking buffer. Samples and standards are incubated in microtiter-plate wells. The second step is to wash the plate and add a second anti-human E-cadherin labelled with peroxidase (POD). During the incubation, human E-cadherin is bound to anti-human E-cadherin (solid phase) on one side and tagged on the other by POD-anti-human E-cadherin. The reaction between POD and substrate (H202 and tetramethylbenzidine) results in colour development with intensities proportional to the amount of human E-cadherin present in samples and standards. The amount of human E-cadherin can be quantitated by measuring the absorbance using an EIA plate reader. Accurate sample concentrations of human E-cadherin can be determined by comparing their specific absorbances with those obtained for the standards plotted on a standard curve.

Storage and Stability: This kit is shipped at 2 - 8C and should be stored at 2 - 8C if not used. Under this condition, the kit is stable until expiration date on label.

Sensitivity and Specificity: This kit specifically measures soluble human E-cadherin. This kit cannot be used to measure mouse E-cadherin. The application of this kit for quantitating E-cadherin from other sources has not been tested

Product Name: Human E-cadherin EIA Kit

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Function: E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Subunit: Homodimer; disulfide-linked. Interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5.

Subcellular Location: Cell junction. Cell membrane; Single-pass type I membrane protein. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm.

Tissue Specificity: Non-neural epithelial tissues.

Ptm: During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions.

Disease: Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis.

Disease: Defects in CDH1 are a cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215].

Disease: Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089].

Similarity: Contains 5 cadherin domains.

Additional Information

Name Human E-cadherin EIA Kit (GWB-40B393)
Related Product Names Human E-cadherin EIA ELISA Kit; E-cadherin; Cadherin-1; Uvomorulin; CAM 120/80; CD324 antigen CDHE; UVOHuman E-cadherin EIA KitCDH1
Swissprot ID P12830
NCBI Acc Number NP_004351.1
Molecular Weight 97456
Datasheets/Manuals Printable datasheet for GWB-40B393
Intended Use Research Use Only