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Mouse ICAM-1 ELISA Kit

GWB-ZZD130

1x96 well plate
$571.20
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Description

Short Description: Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8kDa. As a result of glycosylation, the molecular mass of 110-130KDa is revealed by SDS-PAGE.

Additional Information

Name Mouse ICAM-1 ELISA Kit
Related Product Names BB2| CD 54| CD54| CD54 antigen| Cell surface glycoprotein P3.58| Human rhinovirus receptor| ICAM 1| Intercellular adhesion molecule 1| Major group rhinovirus receptor| MALA2| MGC6195| MyD10| P3.58| Surface antigen of activated B cells
Drywet Surcharge 0
Kit Range 156 pg/ml-10,000 pg/ml
Sensitivity < 10 pg/ml
Sample Type cell culture supernates, serum, plasma(heparin, EDTA) and tissue homogenates
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ICAM1
Gene Fullname intercellular adhesion molecule 1
Gene id 15894
Alias Symbols Intercellular adhesion molecule 1, ICAM1, CD54
Swissprot ID P13597
Protein Name Intercellular adhesion molecule 1
Description of Target Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellula
Kit Principle Genway Biotech ICAM1 ELISA Kit (Mouse) (GWB-ZZD130) is based on standard sandwich enzyme-linked immune-sorbent assay technology. A rat monoclonal antibody specific for ICAM1 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, Q28-N485) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ICAM1 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse ICAM1 captured in well.
Kit Component
ComponentAmount
Lyophilized recombinant mouse Fractalkine standard10 ng/tube x 2
Anti-mouse Fractalkine Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- mouse Fractalkine antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Protein Accession Num NP_034623.1
Nucleotide Accession Num NM_010493.2
Immunogen NSO, Q28-N485
Homology No detectable cross-reactivity with any other cytokine.
Standard Range 156pg/ml-10,000pg/ml. Test has a sensitivity of < 10 pg/ml
Datasheets / Downloads GWB-ZZD130 Datasheet
Specimen/Volumes Refer to Manual
Swiss Prot Number P13597
Specificity There is no detectable cross-reactivity with other relevant proteins.
Concentration Lot Specific
Applications ELISA
GI Number 15894
Stability Four months at 4C and eight months at -20C.
Reconstitution and Storage Store at 4C for frequent use, at -20C for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Reactivity Reactive to Mouse. There is no detectable cross-reactivity with other relevant proteins.
Kit Size 1x96 well plate
Kit Duration ~ 3 Hours
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Intended Use Research Use Only
Key Reference 1. Ballantyne, C. M.; Kozak, C. A.; O'Brien, W. E.; Beaudet, A. L. Assignment of the gene for intercellular adhesion
molecule-1 (Icam-1) to proximal mouse chromosome 9. Genomics 9: 547-550, 1991.
2. Bella, J.; Kolatkar, P. R.; Marlor, C. W.; Greve, J. M.; Rossmann, M. G. The structure of the two amino-terminal
domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand. Proc.
Nat. Acad. Sci. 95: 4140-4145, 1998.
3. Simmons, D.; Makgoba, M. W.; Seed, B. ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell
adhesion molecule NCAM. Nature 331: 624-627, 1988.
Additional Information Range: 156pg/ml-10,000pg/ml
:: Principle: Aviva’s mouse ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse ICAM-1 specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ICAM-1 amount of sample captured in plate.
:: Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 The standard product used in this kit is produced by recombinant gene expression, consisting of 455 amino acids with the molecular mass of 50kDa.