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Clostridium tetani toxin 5S IgG quantitative



1x96 Assays
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Product Name: Clostridium tetani toxin 5S IgG - ELISA

# of Samples: 1 x 96 Assays

Intended Use: The Clostridium tetani toxin 5S IgG-ELISA is intended for the quantitative determination of IgG class antibodies against Clostridium tetani toxin in human serum or plasma (citrate). This allows for the determination of the immune status of the patients facilitating individual recommendations about the necessity of a basic immunization or booster injection.

Introduction: Clostridia are spore-forming gram-positive bacteria. The round spores are build at the terminal end which results in the microscope in a “tennis racket” like shape.
Tetanus develops only when spores of Clostridium tetani germinate under strict anaerobic conditions after gaining access to wounds and small lacerations. The clinical manifestation of the disease is primary not caused by the invasion of the exciter but by the secretion of a powerful neurotoxin (tetanospasmin). This toxin blocks the inhibition of the signal transduction and has a high affinity to the central nervous system. The consequence is hyper excitability of the muscles to external stimuli in combination with a principal increase of the muscle tonus without influence of consciousness. It starts with tonic spasm of muscles (trimus), mimic muscles and gallet muscles. Neck, back and abdominal musculature follow. At the same time the appearance of refectory spasm of whole muscle groups can hamper breathing. Hyper salivation and swallowing problems cause aspiration and pneumonia with the next breath.
Clostridium tetani is ubiquitous present in soil and intestine of humans and animals. Ingestion of bacteria or growth in the intestine of man or animal is without harm. The spores are extremely resistant towards heat and can stay infectious for a long period. The bacteria can get under the skin by even smallest wounds. In Europe tetanus mainly occurs after injuries and sometimes postoperative whereas in developing countries Tetanus is widely disseminated. The WHO assumes that one million people die because of tetanus worldwide per year.
Tetanus toxin is an excellent immunogen in man - only one antigenic type of toxin. The only effective way to control tetanus is by prophylactic active immunization.
Infection may be identified by
Microscopy: Gram stain
Serology: Detection of toxin production by ELISA

Principles of the assay: The quantitative immunoenzymatic determination of IgG-class antibodies against Tetanus toxoid is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiter strip wells are precoated with inactivated Tetanus toxoid antigens to bind coresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured Tetanus toxoid specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of toxoid specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.

Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history, symptomatology as well as serological data.
In immunocompromized patients and newborns serological data only have restricted value.

References Ambrosch,F.,Wiedermann,G.,Müller,H.: Eine neue Mikro-ELISA-Methode zur Bestimmung der Tetanus- Antikörper.Zbl.Bakt.Hyg.A258,173-182 (1984)Mai,K.,Bartelheimer,H.K.,Rosin,H.:Über den Stand und die Dauer des Impfschutzes gegenTetanus bei Kindern.Dtsch.med.Wschr.95,1044 (1970)Müller,H.E.,Müller,M.,Schiek,W.: Tetanus-Schutzimpfung-Indikation und Kontraindikation.Dtsch.med.Wschr.113,1326 (1988)Pietsch,M.:Impfserologie zur Ergänzung von Impfungen.Allgemeinarzt 15,1155-1156 (1993)Schröder,J.P.,Kuhlmann,W.D.: Tetanusimmunität bei Männern und Frauen in der BRD.Immun.Infekt.19,14 (1991)

Additional Information

Name Clostridium tetani toxin 5S IgG quantitative
Related Product Names Clostridium tetani toxin 5S IgG - ELISA
Molecular Weight 0.5
Datasheets / Downloads GWB-1858E0 Datasheet
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.
Intended Use Research Use Only