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In observance of Memorial Day, Genway Biotech office will be closed on Monday, May 28th, 2018

Rat Fibrinogen ELISA Kit

GWB-8088AE

40-374-130015

96 Wells
$355.00
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Description

Short Description: In this assay the Fibrinogen present in serum sample reacts with the anti-Fibrinogen. antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-Fibrinogen. antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum Fibrinogen. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Fibrinogen in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Fibrinogen in the test sample. The quantity of Fibrinogen in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution

Additional Information

Name Rat Fibrinogen ELISA Kit
Related Product Names Rat Fibrinogen ELISA Kit; N/A Rat FibrinogenFGA
Drywet Surcharge 0
Alias Symbols Fibrinogen alpha chain, FGA
Protein Name Fibrinogen alpha chain
Description of Target Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood cl
Swissprot ID P06399
Species Reactivity Rat
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 3.098 ng/ml
Kit Range 25 ng/ml - 800 ng/ml
Homology Rat
NCBI Acc Number NP_000499.1
Standard Range Refer To Manual
Sample Type Serum, plasma and other biological fluids
Product Format In Solution
Datasheets / Downloads GWB-8088AE Datasheet
Specimen/Volumes Refer To Manual
Swiss Prot Number P02671
Applications ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-FIB conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-RAT FIBRINOGEN ELISA MICRO PLATE
Anti-Rat FIB coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. RAT FIBRINOGEN CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Rat
Kit Size 96 well
Kit Principle Genway Biotech Fibrinogen ELISA Kit (Rat) (GWB-8088AE) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Fibrinogen alpha chain has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Fibrinogen alpha chain - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fibrinogen alpha chain captured in the well.
Kit Component
ComponentAmount
Anti-Rat Fibrinogen Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat Fibrinogen Calibrator1
Kit Duration 100 Minutes
Storage Refer to kit instructions for component storage
Intended Use Research Use Only
Additional Information Intended Use: The Fibrinogen test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of rats.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat Fibrinogen antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT FIBRINOGEN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat FIB.
7. RAT FIBRINOGEN CALIBRATOR
One vial containing Rat Fibrinogen calibrator.