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Cytomegalovirus (CMV) IgG Enzyme Immunoassay Test Kit



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Species Human.
Sample Type Serum. For the determination of Human Cytomegalovirus (CMV) in serum. DNA Virus Infections, Infection, Blood, RNA Virus Infections, Pathologic Processes.
Product Name Cytomegalovirus (CMV).
Sample Type Serum.
# of Samples 1X96 Assays.
Intended Use The CMV IgG ELISA is intended for use in evaluating a patient's serologic status to cytomegalovirus (CMV) infection.
Introduction Cytomegalovirus is a herpes virus and a leading biological factor causing congenital abnormalities and complications among those who receive massive blood transfusions and immunosuppressive therapy. About half the pregnant women who contract a primary infection spread the disease to their fetus. When acquired in-utero, the infection may cause mental retardation, blindness, and/or deafness. Serological tests for detecting the presence of antibody to CMV can diagnose active or recent infection and provide valuable information regarding the history of previous infection. These tests are also useful in screening blood for transfusions in newborns and immuno-compromised recipients. GenWay Biotech CMV IgG ELISA is an accurate serologic method to detect CMV IgG antibody for identification of CMV infection.
Principle of the test Purified CMV antigen is coated on the surface of microwells. Diluted patient serum is added to the wells, and the CMV IgG specific-antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of CMV IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrators and controls.
Limitation of the Test 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice.
2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
3. Serum samples demonstrating gross lipemia, gross hemolysis, or turbidity should not be used with this test.
4. The results obtained from the use of this kit should be used only as an adjunct to other diagnostic procedures and information available to the physician.
Precision A study was performed to document typical assay precision with the GenWay Biotech CMV IgG EIA product. The mean, SD, and %CV were calculated for Intra-and Inter-Assay in concentration value (IU/mL).
Product Name Cytomegalovirus (CMV).
Protocols from Publications Citation Wang GC, Kao WH, Murakami P, Xue QL, Chiou RB, Detrick B, McDyer JF, Semba RD, Casolaro V, Walston JD, Fried LP. Cytomegalovirus infection and the risk of mortality and frailty in older women: a prospective observational cohort study. Am J Epidemiol. 2010 May 15;171(10):1144-52. Epub 2010 Apr 16. PubMed PMID: 20400465; PubMed Central PMCID: PMC2877470.
Experiment Name CMV antibody measurement.
Experiment Background CMV antibody concentration would be associated with a higher risk of frailty and mortality.
Experiment Steps Measure CMV IgG concentration in stored (-70°C) baseline serum using a commercial enzyme-linked immunosorbent assay kit (GenWay Biotech, San Diego, California).
Product Protocol To see this product used in a peer reviewed journal, click the following link
Cytomegalovirus Infection and the Risk of Mortality and Frailty in Older Women: A Prospective Observational Cohort Study

Additional Information

Name Cytomegalovirus (CMV) IgG Enzyme Immunoassay Test Kit
Related Product Names CMV3; Cytomegalovirus (CMV) ELISA Kit Cytomegalovirus (CMV)CMV
Datasheets / Downloads GWB-892399 Datasheet
Intended Use Research Use Only