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Cytomegalovirus (CMV) IgM



96 Wells
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Product Name: Cytomegalovirus IgM ELISA

# of Samples: 1 x 96 Assays

Intended Use: The Cytomegalovirus IgM ELISA is intended for the qualitative determination of IgM class antibodies against CMV in human serum or plasma (citrate).

Introduction: Cytomegalovirus (CMV) is a member of the herpesvirus group (Betasubfamily, DNA virus of 150-200 nm). These viruses share a characteristic ability to remain dormant within the body over a long period. Initial CMV infection, which may have few symptoms, is always followed by a prolonged, inapparent infection during which the virus resides in cells without causing detectable damage or
clinical illness. Severe impairmant of the body´s immune system by medication or disease consistently reactivates the virus from the latent or dormant state.
CMV is found universally throughout all geographic locations and socioeconomic groups, and infects between 50% and 85% of adults. CMV infection is more widespread in developing countries and in areas of lower socioeconomic conditions. For the vast majority of people, CMV infection is not a serious problem, but it is to certain high-risk groups:
the unborn baby during pregnancy
people who work with children
immunocompromised persons, such as organ transplant recipients and persons infected with HIV
The presence of virus resp. infection may be identified by
Serology: CBR, Detection of antibodies by ELISA

Principles of the assay: The qualitative immunoenzymatic determination of IgM-class antibodies against CMV is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiter strip wells are precoated with CMV antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured CMV-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of CMV specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

Storage and Stability: The reagents are stable up to the expiry date when stored at 2...8 °C.

References: Maine GT, Lazzarotto T, Landini MP. New developments in the diagnosis of maternal and congenital CMV infection. Expert Rev Mol Diagn. 2001 May;1(1):19-29.Mezzasoma L, Bacarese-Hamilton T, Di Cristina M, Rossi R, Bistoni F, Crisanti A.Antigen microarrays for serodiagnosis of infectious diseases. Clin Chem. 2002 Jan;48(1):121-30.Numazaki K. Cytomegalovirus infection. Nippon Rinsho. 2001 Nov;59 Suppl 7:112...8.Zaia JA, Sissons JG, Riddell S, Diamond DJ, Wills MR, Carmichael AJ, Weekes MP, Gandhi M, Rosa CL, Villacres M, Lacey S, Markel S, Sun J. Status of Cytomegalovirus Prevention and Treatment in 2000. Hematology (Am Soc Hematol Educ Program). 2000:339-355.Weber B, Berger A, Rabenau H.Human cytomegalovirus infection: diagnostic potential of recombinant antigens for cytomegalovirus antibody detection. J Virol Methods. 2001 Aug;96(2):157-70.

Additional Information

Name Cytomegalovirus (CMV) IgM
Related Product Names Cytomegalovirus IgM – ELISA
Molecular Weight 0.5
Storage The reagents are stable up to the expiry date when stored at 2...8 °C.
Datasheets/Manuals Printable manual for GWB-3E8402
Intended Use Research Use Only