Rat Hemopexin ELISA Kit

GWB-20B00B

40-374-130018

96 Wells
$400.00
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Description

Short Description: In this assay the Hemopexin present in serum sample reacts with the anti-HX antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP), are added. These enzymelabeled antibodies form complexes with the previously bound serum HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemopexin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemopexin in the test sample. The quantity of Hemopexin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.

Additional Information

Name Rat Hemopexin ELISA Kit
Related Product Names Rat Hemopexin ELISA Kit; Beta-1B-glycoprotein Rat HemopexinHpx
Drywet Surcharge 0
Kit Range 6.25 ng/ml - 400 ng/ml
Sensitivity 1.358 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol HPX
Gene Fullname hemopexin
Gene id 58917
Alias Symbols HPX, Hemopexin, Beta-1B-glycoprotein, HPXN
Swissprot ID P20059
Protein Name Hemopexin
Description of Target Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Kit Principle Genway Biotech Hemopexin ELISA Kit (Rat) (GWB-20B00B) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Hemopexin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Hemopexin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hemopexin captured in the well.
Kit Component
ComponentAmount
Anti-Rat Hemopexin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat Hemopexin Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Rat
NCBI Acc Number NP_000604.1
Standard Range Refer To Manual
Product Format In Solution
Datasheets / Downloads GWB-20B00B Datasheet
Specimen/Volumes Refer To Manual
Swiss Prot Number P02790
Applications ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-HX conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-RAT HEMOPEXIN ELISA MICRO PLATE
Anti-Rat HX coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. RAT HEMOPEXIN CALIBRATOR
The lyophilized Rat Hemopexin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Rat
Kit Size 96 well
Kit Duration 80 Minutes
Storage Refer to kit instructions for component storage
Intended Use Research Use Only
Additional Information Intended Use: The Hemopexin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemopexin in biological samples of rats.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat Hemopexin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT HEMOPEXIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat HX.
7. RAT HEMOPEXIN CALIBRATOR
One vial containing a lyophilized Rat Hemopexin calibrator.