Description
Amino SequenceFull length Activity: Not known
Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 61 kDa (Including GST). The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3\' of the DNA lesion while the ERCC1-XPF complex makes the 5\' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 61 kDa (Including GST). The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3\' of the DNA lesion while the ERCC1-XPF complex makes the 5\' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
Additional Information
| Name | ERCC1 (GWB-122D21) |
|---|---|
| Related Product Names | RECOMBINANT HUMAN ERCC1 PROTEIN ERCC1 |
| Swissprot ID | P07992 |
| Source | Recombinant protein encoding human full length ERCC1 expressed i |
| Application | ELISA, IA, WB |
| Format | 100ul purified human ERCC1 Protein At 100ug/ ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol. |
| Datasheets/Manuals | Printable datasheet for GWB-122D21 |
| Storage | Store vial at -20C to -80C. When stored at the recommended temperature, this protein is stable for 12 months. |
| Intended Use | Research Use Only |