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Dehydroepiandrosterone-Sulfate ELISA Kit



1x96 well plate
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Product Name Dehydroepiandrosterone-Sulfate
Category Fertility.
Sample Type Serum and plasma.
Sensitivity 0.044 ug/mL.
Description DHEA-S Enzyme Immunoassay Kit provides materials for the Quantitative Determination of DHEA-S in serum and plasma.
Introduction The GenWay DHEA-S Enzyme Immunoassay Kit provides materials for the quantitative determination of DHEA-S in serum and plasma.
This assay is intended for research use only.
Dehydroepiandrosterone (5-Androstene-3β-0L-17-one, Androstenolone, Dehydroisoandrosterone, Transdehydroandrosterone, DHEA) is a steroid hormone present in blood mostly in its sulfate form (DHEA-S).
DHEA-S is a more specific product of the adrenals and measurements of this steroid are widely used in clinical practice. The clinical importance of plasma assays of DHEA-S is associated with the diagnosis of adrenal hyperplasia and differential diagnosis of hirsutism (1).
Principle of the Test The GenWay DHEA-S ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards a unique antigenic site of the DHEA-S molecule.
Endogenous DHEA-S of a patient sample competes with a DHEA-S horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off.
The amount of bound peroxidase conjugate is reverse proportional to the concentration of DHEA-S in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of DHEA-S in the patient sample.
Storage and Stability When stored at 2°C-8°C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
All opened reagents must be stored at 2°C-8°C. Microtiter wells must be stored at 2°C-8°C. Once the foilbag has been opened, care should be taken to close it tightly again.
Function Catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands.
Catalytic Activity 3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
3'-phosphoadenylyl sulfate + taurolithocholate = adenosine 3',5'-bisphosphate + taurolithocholate sulfate.
Subunit Homodimer.
Subcellular Location Cytoplasm.
Tissue Specificity Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.
Ptm The N-terminus is blocked.
Miscellaneous Estrogens present in maternal circulation is predominantly derived from fetal dehydroepiandosterone sulfate which is hydrolyzed and metabolized to estrogens in placenta.
Similarity Belongs to the sulfotransferase 1 family.

Additional Information

Name Dehydroepiandrosterone-Sulfate ELISA Kit
Related Product Names Dehydroepiandrosterone-Sulfate ELISA Kit; EC; Hydroxysteroid Sulfotransferase; HST; Dehydroepiandrosterone sulfotransferase; DHEA-ST; ST2; ST2A3 HST; STDDehydroepiandrosterone-SulfateDHEA-S
Swissprot ID Q06520
NCBI Acc Number NP_003158.2
Molecular Weight 33780
Datasheets/Manuals Printable manual for GWB-46B3CF
Intended Use Research Use Only