Progesterone Saliva ELISA



96 Wells
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Product Name: Progesterone Saliva

# of Samples: 1 x 96 Assays

Intended Use: Competitive immunoenzymatic colorimetric method for quantitative determination of Progesterone in saliva.

Introduction: Progesterone is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans and other species. Progesterone is the major naturally occurring human progestagen.
Progesterone is important for aldosterone (mineralocorticoid) synthesis, as 17-hydroxyprogesterone is for cortisol (glucocorticoid).
Progesterone levels are relatively low in children and postmenopausal women. Adult males have levels similar to those in women during the follicular phase of the menstrual cycle.
In women, progesterone levels are relatively low during the preovulatory phase of the menstrual cycle, rise after ovulation, and are elevated during the luteal phase. If pregnancy occurs, progesterone levels are maintained at luteal levels initially. After delivery of the placenta and during lactation, progesterone levels are very low. The fall in progesterone levels following delivery is one of the triggers for milk production .
Progesterone is produced in the adrenal glands, the gonads (specifically after ovulation in the corpus luteum), the brain, and, during pregnancy, in the placenta.
Progesterone converts the endometrium to its secretory stage to prepare the uterus for implantation. If pregnancy does not occur, progesterone levels will decrease, leading, in the human, to menstruation.
Progesterone belongs to the group of neurosteroids that are found in high concentrations in certain areas in the brain and are synthesized there. Neurosteroids affect synaptic functioning, are neuroprotective, and affect myelinization.
Progesterone has multiple effects outside of the reproductive system. Progesterone is thermogenic, it reduces spasm and relaxes smooth muscle. Bronchi are widened and mucus regulated. Progesterone acts as an antiinflammatory agent and regulates the immune response. Progesterone also assists in thyroid function, in bone building by osteoblasts.
Measurement of serum progesterone concentrations have been used in evaluating ovarian function

Principles of the assay: Microtiter strip wells are precoated with anti-Progesterone antibodies (solid-phase). Progesterone in the sample competes with added horseradish peroxidase labelled Progesterone (enzyme-labelled antigen) for antibody binding. After incubation a bound/free separation is performed by solid-phase washing. The immune complex formed by enzyme-labelled antigen is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is inversely proportional to the amount of Progesterone in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorption at 450 nm is read using an ELISA microwell plate reader.

Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the dark.

Limitations of the Test: Sample(s), which are contaminated microbiologically, should not be used in the assay. It is important that the time of reaction in each well is held constant for reproducible results. Pipetting of samples should not extend beyond ten minutes to avoid assay drift. If more than one plate is used, it is recommended to repeat the dose response curve. Addition of the substrate solution initiates a kinetic reaction, which is terminated by the addition of the stop solution. Therefore, the addition of the substrate and the stopping solution should be added in the same sequence to eliminate any time deviation during reaction. Plate readers measure vertically. Do not touch the bottom of the wells. Failure to remove adhering solution adequately in the aspiration or decantation wash step(s) may result in poor replication and spurious results.

References Wisdom G.B. (1976) Clin. Chem. 22 (8), 1243 – 1255.De Villa G.O. et al. (1972) J. Clin. Endoc. Metab. 35, 458.Joyce B.G., Graham F.R. and Fahmy D.R. (1977) Steroids 29 (6), 761.Winkel P., Gaede P. and Lyngbye J. (1976) J. Clin. Chem. 22 (4), 422.Rajkowski K.N., Cittanova N., Desfosses B. and Jayle M.F. (1977) Steroids 29 (5), Lu Y., Bentley G.R. and Gann P. (1999) Fertility and Sterility 71 (5), 863.

Additional Information

Name Progesterone Saliva ELISA
Related Product Names Progesterone Saliva ELISA
Molecular Weight 0.5
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the dark.
Datasheets/Manuals Printable manual for GWB-373DFD
Intended Use Research Use Only