Sperm-Antibody ELISA Kit (GWB-D94B59)



1x96 well plate


Product Name: Sperm-Antibody

Category: Fertility

Sample Type: Serum and Seminal Plasma

Description: Reliable and quantitative test for the determination antibodies directed against human spermatozoa. This test is intended for the use with serum.

Introduction: The anti-spermatozoa antibody ELISA from GenWay is a reliable and quantitative test for the determination antibodies directed against human spermatozoa. This test is intended for the use with serum. Antibodies directed against spermatozoa antigens may cause infertility in women or men. Unwanted childlessness is a growing problem with which up to 20% of all couples in the reproductive age are confronted temporarily or longterm. In 20% of these cases the presence of anti-spermatozoa antibodies in the male or the female sample is detectable (1).
The definition of infertility according to the WHO (WHO Laboratory Manual for the Examination of Human Semen and Semen Cervical- Mucus Interaction, 1999) is the absence of a conception within 12 months of unprotected intercourse. The main cause of an immunological fertility disorder is the formation of antibodies directed against spermatozoa antigens. Anti-spermatozoa antibodies exert heterogeneous effects on the ability of spermatozoa to fertilize. The inhibiting effect of anti-spermatozoa antibodies on the motility of spermatozoa by binding to their surface and by agglutinating processes is well-known (2).
The penetration of the spermatozoa into the cervical mucus is impaired by the presence of anti-spermatozoa antibodies in the seminal plasma and/or in the cervical mucus (3)
Anti-spermatozoa antibodies negatively influence the capacitation and the acrosome reaction of spermatozoa and thereby impede the interaction of the spermatozoa with the oocyte (4).
The interaction of the spermatozoon with the oocyte and the subsequent binding to and penetration of the zona pellucida may be inhibited by anti-spermatozoa antibodies. The following fusion of the oocyte and a spermatozoon may also be impaired by the presence of antispermatozoa antibodies (5).
According to Crosignani et al. (6), the rate of pregnancies in couples with anti-spermatozoa antibodies on the part of the man or the woman are 38% lower compared to the control groups. Furthermore an influence on the implantation and on the early embryological development could be confirmed. An association of anti-spermatozoa antibodies and miscarriages is discussed. The frequency of anti-spermatozoa antibodies in infertile couples amounts to 20% (7).
Anti-spermatozoa antibodies may occur dissolved in the ejaculate or bound to the surface of spermatozoa. Anti-spermatozoa antibodies may be found in men and in women (8). In women anti-spermatozoa antibodies may be found in cervical mucus, oviduct liquid and follicular liquid. Men having more than 50% of their spermatozoa coated with anti-spermatozoa antibodies show a conspicuously reduced rate of fertility (9).

Principle of the test: The anti-spermatozoa antibody ELISA (Enzyme Linked ImmunoSorbent Assay) from G e n W a y is a solid-phase sandwich enzymeimmunoassay for the quantitative determination of anti-spermatozoa antibodies in human serum. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa antibodies. The samples and calibrators are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of anti-human globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3\',5,5\'-tetramethylbenzidine) yielding a colour reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength >550 nm is recommended.

Storage and Stability: When stored at 2 to 8C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
Enzyme-Conjugate, Substrate Solution, Calibrators and Zero Calibrator must be stored at 2 to 8C.
Microtiter wells must be stored at 2 to 8C. Once the foilbag has been open care should be taken to close it tightly again, with dessicant. In these conditions, they are stable at least 4 weeks after opening of the sealed bag.

Additional Information

Name Sperm-Antibody ELISA Kit (GWB-D94B59)
Related Product Names Sperm-Antibody ELISA Kit Sperm-Antibody
Datasheets/Manuals Printable manual for GWB-D94B59
Intended Use Research Use Only