Leptin (Ms/Rat) ELISA Kit

GWB-69C0CF

40-056-205065

1x96 well plate
$921.50
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Description

Product Name: Leptin (Ms/Rat)

Category: Diabetes & Metabolism

Sample Type: Serum and plasma

Sensitivity: 10 pg/mL

Description: Enzyme Immunoassay for Quantitative Determination of Mouse and Rat Leptin (Obese Protein).

# of Samples: 1X96 Assays

Introduction: Leptin, the product of the ob gene (1,2), is a recently discovered single-chain proteohormone with a molecular weight of 16 kD which is thought to play a key role in the regulation of body weight. Its amino acid sequence exhibits no major homologies with other proteins (1). Leptin is almost exclusively produced by differentiated adipocytes (3-5). It acts on the central nervous system, in particular the hypothalamus, thereby suppressing food intake and stimulating energy expenditure (2,6-9). Leptin receptors - alternatively spliced forms exist that differ in length - belong to the cytokine class I receptor family (10-12). They are found ubiquitously in the body (10,11,13,14) indicating a general role of leptin which is currently not fully understood. A circulating form of the leptin receptor exists which acts as one of several leptin binding proteins (15).
Besides its metabolic effects, leptin was shown to have a strong influence on a number of endocrine axes. In male mice, it blunted the starvation-induced marked decline of LH, testosterone, thyroxine and the increase of ACTH and corticosterone. In female mice, leptin prevented the starvation-induced delay in ovulation (16). Ob/ob mice, which are leptin deficient due to an ob gene mutation, are infertile. This defect could be corrected by administration of leptin, but not through weight loss due to fasting (17), suggesting that leptin is pivotal for reproductive functions.
All these actions may, at least in part, be explained by the suppressive effect of leptin on neuropeptide Y (NPY) expression and secretion by neurons in the arcuate nucleus (6,18,19). NPY is a strong stimulator of appetite (20,21) and is known to be involved in the regulation of various pituitary hormones, e.g. suppression of GH through stimulation of somatostatin (22,23), suppression of gonadotropins (23) or stimulation of the pituitary-adrenal axis (21).
The most important variable that determines circulating leptin levels is body fat mass (24-26). Obviously, under conditions of regular eating cycles, leptin reflects the proportion of adipose tissue (27) showing an exponential relationship (37). This constitutive synthesis of leptin is modulated by a number of non-hormonal and hormonal variables. Stimulators in both rodents and humans are overfeeding (28,29), insulin (3,5,30-33) and glucocorticoids (5,34-36). Suppression has been shown for fasting (27), cAMP and beta--3-adrenoceptor agonists (35). From these findings it becomes clear that leptin is an integral component of various metabolic and endocrine feedback loops (38).

Technical Features +Applications: - Highly specific, sensitive and fast assay for mouse and rat leptin
- Calibrated against the WHO International Standard for mouse leptin: Code 97/626 (39, 40)
- No extraction required - No interference from leptin binding proteins due to high affinity of antiserum
- Complete recovery, measures total leptin in serum
- High sensitivity allows precise measurement also in lean animals: Detection limit 10 pg/ml
- Small sample volume requirement due to high sensitivity: e.g., less than 5 ?l/well

Product Name: Leptin (Ms/Rat)

Function:
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Subunit: Interacts with SIGLEC6.

Subcellular Location: Secreted.

Disease: Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].

Similarity: Belongs to the leptin family.

Additional Information

Name Leptin (Ms/Rat) ELISA Kit
Related Product Names Leptin (Ms/Rat) ELISA Kit; Obesity factor; Obese protein OB; OBSLeptin (Ms/Rat)LEP
NCBI Acc Number NP_000221.1
Molecular Weight 18641
Datasheets / Downloads Click here to download product manual.
Swiss Prot Number P41159
Intended Use Research Use Only