Herpes simplex Virus 1+2 (HSV) IgG mixed antigens



96 Wells
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Product Name Herpes Simplex Virus 1+2 (HSV) IgG - ELISA.
# of Samples 1 x 96 Assays.
Intended Use The HSV-1+2 IgG-ELISA is intended for the qualitative determination of IgG class antibodies against Herpes simplex virus Type 1+2 in human serum or plasma (citrate).
Introduction Herpes simplex is an enveloped DNA virus (150-200 nm in diameter) belonging to the alpha-herpesviridae. Based on antigenic, biochemical and biologically differences it can be divided into two serotypes, HSV-1 and HSV-2. Man is the only known natural host and source of the virus. HSV Type 1 typically causes oral herpes, while HSV-type 2 typically affects the genital area. Most of the time, HSV-1 and HSV-2 are inactive, or silent, and cause no symptoms, but some infected people have outbreaks of blisters and ulcers. Once infected with HSV, people remain infected for life. Herpes simplex viruses are amongst the most common infectious agents of man, and either HSV type appears to be capable of infecting similar body sites. A high percentage of the adult population is seropositive (appr. 90% HSV-1, in dependence on the socioeconomic status 10-30% HSV-2). Primary HSV-1 infection usually occurs in early childhood (6 to 18 months of age). HSV-2 usually produces mild symptoms, and most people have no recognized symptoms. Persons at risk are children with inherited T-cell deficiencies and patients who are immunosuppressed because of infection (e.g. HIV), transplantation, or cancer therapy.
The presence of virus resp. infection may be identified by
  • Microscopy: CPE, IF
  • PCR.
  • Serology: Detection of antibodies by ELISA.
Principles of the assay The qualitative immunoenzymatic determination of IgG-class antibodies against HSV Type 1+2 is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with HSV Type 1+2 antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured HSV Type 1+2-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of HSV Type 1+2-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Storage and Stability The reagents are stable up to the expiry date stated on the label when stored at 2-8°C.
Limitations of the Test Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. The presence of IgG antibodies to HSV 1+2 do not imply a protection for a further HSV 1+2 infection. Crossreactivity with VZV cannot be excluded. A significant high HSV antibody titer can be caused by a VZV infection and vice versa. A precise diagnosis should take into consideration clinical history, symptomatology as well as serological data. In immunocompromised patients and newborns serological data only have restricted value.
References Pass, R.F., R.J. Whitley, J.D. Whelchel, A.G. Diethelm, D.W. Reynolds, and C.A. Alford. 1979. Identification of Patients with Increased Risk of Infection with HSV after Renal Transplantation. J. Infect. Dis. 140:487-492.Meyer, J.D., N. Fluornoy, and E.D. Thomas. 1980. Infection with HSV and Cell Mediated Immunity after Bone Marrow Transplant. J. Infect. Dis. 142:338-346 Hutfield, D.C. 1966. History of Herpes Genitalis. J. of Vener. Dis. 42:263-268Gentry, G.A., and C.C. Randall. 1973. The Physical and Chemical Properties of the HSV. In: The HSV. A.S. Kaplan, ed. New York Academic Press. P. 45.Whitney, R.J., Nahmias, et al. 1980. The Natural History of HSV Infection of Mother and Newborn. Pediatrics. 66:489-494.

Additional Information

Name Herpes simplex Virus 1+2 (HSV) IgG mixed antigens
Related Product Names Herpes Simplex Virus 1+2 (HSV) IgG - ELISA
Molecular Weight 0.5
Datasheets / Downloads Click here to download product manual.
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.
Intended Use Research Use Only