Herpes simplex Virus 1+2 (HSV) IgM mixed antigens (GWB-3F02FE)



96 Wells
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Product Name: Herpes Simplex Virus 1+2 (HSV) IgM - ELISA

# of Samples: 1 x 96 Assays

Intended Use: The HSV-1+2 IgM-ELISA is intended for the qualitative determination of IgM class antibodies against Herpes simplex virus Type 1+2 in human serum or plasma (citrate).

Introduction: Herpes simplex is an enveloped DNA virus (150-200 nm in diameter) belonging to the alpha-herpesviridae. Based on antigenic, biochemical and biologically differences it can be divided into two serotypes, HSV-1 and HSV-2. Man is the only known natural host and source of the virus. HSV Type 1 typically causes oral herpes, while HSV-type 2 typically affects the genital area. Most of the time, HSV-1 and HSV-2 are inactive, or “silent”, and cause no symptoms, but some infected people have “outbreaks” of blisters and ulcers. Once infected with HSV, people remain infected for life. Herpes simplex viruses are amongst the most common infectious agents of man, and either HSV type appears to be capable of infecting similar body sites. A high percentage of the adult population is seropositive (appr. 90% HSV-1, in dependence on the socioeconomic status 10-30% HSV-2). Primary HSV-1 infection usually occurs in early childhood (6 to 18 months of age). HSV-2 usually produces mild symptoms, and most people have no recognized symptoms. Persons at risk are children with inherited T-cell deficiencies and patients who are immunosuppressed because of infection (e.g. HIV), transplantation, or cancer therapy.
The presence of virus resp. infection may be identified by
Microscopy: CPE, IF
Serology: Detection of antibodies by ELISA

Principles of the assay: The qualitative immunoenzymatic determination of IgM-class antibodies against HSV Type 1+2 is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with HSV Type 1+2 antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured HSV Type 1+2-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of HSV Type 1+2-specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2...8 C.

References: Pass, R.F., R.J. Whitley, J.D. Whelchel, A.G. Diethelm, D.W. Reynolds, and C.A. Alford. 1979. Identification of Patients with Increased Risk of Infection with HSV after Renal Transplantation. J. Infect. Dis. 140:487-492.Meyer, J.D., N. Fluornoy, and E.D. Thomas. 1980. Infection with HSV and Cell Mediated Immunity after Bone Marrow Transplant. J. Infect. Dis. 142:338-346Hutfield, D.C. 1966. History of Herpes Genitalis. J. of Vener. Dis. 42:263-268Gentry, G.A., and C.C. Randall. 1973. The Physical and Chemical Properties of the HSV. In: The HSV. A.S. Kaplan, ed. New York Academic Press. P. 45.Whitney, R.J., Nahmias, et al. 1980. The Natural History of HSV Infection of Mother and Newborn. Pediatrics. 66:489-494.

Additional Information

Name Herpes simplex Virus 1+2 (HSV) IgM mixed antigens (GWB-3F02FE)
Availability In Stock
Related Product Names Herpes Simplex Virus 1+2 (HSV) IgM - ELISA
Molecular Weight 0.5
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 C.
Datasheets/Manuals Printable manual for GWB-3F02FE
Intended Use Research Use Only