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Herpes simplex Virus 2 (HSV 2) IgG recombinant antigens



1x96 Assays
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Product Name Herpes Simplex Virus 2 (HSV 2) IgG - ELISA.
# of Samples 1 x 96 Assays.
Intended Use The HSV 2 IgG recombinant ELISA allows the detection of HSV 2 infection in presence of antibodies to HSV 1 in human serum or plasma (citrate).
Introduction Herpes simplex is an enveloped DNA virus (150-200 nm in diameter) belonging to the alpha-herpesviridae. Based on antigenic, biochemical and biologically differences it can be divided into two serotypes, HSV-1 and HSV-2. Man is the only known natural host and source of the virus. HSV type 1 typically causes oral herpes, while HSV type 2 typically affects the genital area. Most of the time, HSV-1 and HSV-2 are inactive, or “silent”, and cause no symptoms, but some infected people have “outbreaks” of blisters and ulcers. Once infected with HSV, people remain infected for life. Herpes simplex viruses are amongst the most common infectious agents of man, and either HSV type appears to be capable of infecting similar body sites. A high percentage of the adult population
is seropositive (appr. 90% HSV-1, in dependence on the socio-economic status 10-30% HSV-2). Primary HSV-1 infection usually occurs in early childhood (6 to 18 months of age). HSV-2 usually produces mild symptoms, and most people have no recognized symptoms. Persons at risk are children with inherited T-cell deficiencies and patients who are immunosuppressed because of infection (e.g. HIV), transplantation, or cancer therapy.
HSV-2 can cause potentially fatal infections in infants if the mother is shedding virus at the time of delivery. - HSV-2 may play a major role in the heterosexual spread of HIV: herpes can make people more susceptible to HIV infection, and HIV-infected individuals more infectious.
The presence of virus resp. infection may be identified by
Microscopy: CPE, IF
Serology: Detection of antibodies by ELISA.
Principles of the assay Recombinant gG2 protein of HSV-2 show diffuse band in SDS electrophoresis corresponding to gG2 which is found at approximately 43 kD. This antigen will allow the detection of HSV 2 infection in the presence of antibodies to HSV 1. This is important as the % of the population with HSV 1 increases throughout life up to >90 % at old age. HSV 2 is generally sexually transmitted and in all societies is associated with the amount of sexual activity and number of partners. Genital herpes is important to diagnose in pregnancy as sheeding virus can lead to congenital infection of the child witch is invariably serious. The qualitative immunoenzymatic determination of IgG-class antibodies against HSV Type 2 is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiter strip wells are precoated with HSV Type 2 recombinant antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured HSV Type 2 specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of HSV Type 2 specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Storage and Stability The reagents are stable up to the expiry date stated on the label when stored at 2..8°C.
Limitations of the Test Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history, symptomatology as well as serological data. In immunocompromised patients and newborns serological data only have restricted value.
References Corey, L.; P.G. Spear: Infections with herpes simplex viruses. N. Engl. J. Med. 314 (1986) 686-691Dörries, R., R. Kaiser, H. Imrich et al.: Neuere Aspekte zur Diagnose zentralnervöser Virusinfektionen. Lab. Med. 15 (1991) 99-102Felgenhauer, K.: Diagnostische Bedeutung der lokal synthetisierten spezifischen Antikörper des Liquor cerebrospinalis. Lab. Med. 15 (1991) 208 Kaufmann, H.E.;F. Martola and C. Dohlman: The use of 5-iodo-2’-deoxy-uridine (IDU) in the treatment of herpes simplex keratitis. Arch. Ophthal. N. Y. 68 (1962) 235-239Spaar, F.-W.: Die menschliche Herpes simplex Enzephaltits und –Meningitis. Eine klinisch-neurologische Untersuchung. G. Fischer Verlag, Stuttgart, New York (1976)

Additional Information

Name Herpes simplex Virus 2 (HSV 2) IgG recombinant antigens
Related Product Names Herpes Simplex Virus 2 (HSV 2) IgG - ELISA
Molecular Weight 0.5
Datasheets / Downloads GWB-6271A3 Datasheet
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.
Intended Use Research Use Only