Human Serum Amyloid A (SAA) ELISA

96 well
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In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Additional Information

Name Human Serum Amyloid A (SAA) ELISA
Related Product Names SAA, Serum amyloid A protein
Standard Range Refer To Manual
Sample Type Refer To Manual
Kit Size 96 well
Specimen/Volumes Refer To Manual
Intended Use Research Use Only
NCBI Acc Number P35541.2
GI Number 143811456
Storage Refer to kit instructions for component storage
Swiss Prot Number P02735 (SAA_HUMAN)
Applications ELISA