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Helicobacter Pylori IgG Enzyme Immunoassay Test Kit

GWB-504F20

40-052-115023

ELISA_Kits
$316.80
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Description

Species: Human

Sample Type: Serum

For the quantitative determination of IgG Antibodies to Helicobacter pylori in serum. Bacterial Infections, Infection, Blood, Gastrointestinal Diseases, Gastrointestinal Tract Product Name: IgG Antibodies to Helicobacter pylori

Sample Type: Serum

# of Samples: 1X96 Assays

Intended Use: The Helicobacter pylori IgG Test Kit is intended for use in evaluating the serologic status to H. pylori infection in specimens with gastrointestinal symptoms.

Introduction: Helicobacter pylori is a spiral bacterium cultured from human gastric mucosa discovered by B.J. Marshall in 1982. Studies have indicated that the presence of H. pylori is associated with a variety of gastrointestinal diseases including gastritis, duodenal and gastric ulcers, non-ulcer dyspepsia, and gastric adenocarcinoma and lymphoma. The studies have also demonstrated that removal of the organism by anti-microbial therapy is correlated with the resolution of symptoms and cure of diseases

Principle of the test: Purified H. pylori antigen is coated on the surface of microwells. Diluted samples serum is added to the wells, and the H. pylori IgG- specific antibody, if present, binds to the antigen. All unbound materials are washed away. Enzyme conjugate is added, which binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and substrate and chromogen are added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.

Storage and Stability: 2-8C

Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice.
2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
3. Serum samples demonstrating gross lipemia, gross hemolysis, or turbidity should not be used with this test.

Precision: A study was performed to document typical assay precision with the GenWay Biotech H. pylori IgG EIA product. The mean, SD, and %CV were calculated for Intra- and Inter-Assay in O.D. value (A450).

Product Name: IgG Antibodies to Helicobacter pylori

Subcellular Location:
Secreted.

Disease: Chromosomal aberrations involving IGHG1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.

Miscellaneous: Nie has the G1M(17) allotypic marker, 97-K, and the G1M(1) markers, 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

Miscellaneous: Nie also differs in the amidation states of 35, 116, 198, 269 and 272.

Miscellaneous: EU also differs in the amidation states of residues 155, 166, 177, 195, 198, 269, and 272 and in the order of residues 268-272.

Miscellaneous: KOL also differs in the amidation states of residues 198, 267 and 272.

Additional Information

Name Helicobacter Pylori IgG Enzyme Immunoassay Test Kit
Related Product Names IgG Antibodies to Helicobacter pylori ELISA Kit; N/A IgG Antibodies to Helicobacter pyloriIGHG1
Molecular Weight 36106
Datasheets / Downloads GWB-504F20 Datasheet
Swiss Prot Number P01857
Intended Use Research Use Only