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Human MMP-9 ELISA Kit

GWB-ZZD204

1x96 well plate
$614.04
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Description

Short Description: The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.

Additional Information

Name Human MMP-9 ELISA Kit
Related Product Names 82 kDa matrix metalloproteinase-9|92 kDa gelatinase|92 kDa type IV collagenase|CLG4B|Collagenase Type 4 beta|Collagenase type IV 92 KD|EC 3.4.24.35|Gelatinase 92 KD|Gelatinase B|Gelatinase beta|GelatinaseB|GELB|Macrophage gelatinase|MANDP2|Matrix metallop
Drywet Surcharge 0
Kit Range 156 pg/mL-10,000 pg/mL
Sensitivity < 5 pg/mL
Sample Type cell culture supernates, serum, plasma(heparin), saliva and urine
Kit Detection Colorimetric, OD 450 nm
Gene Symbol MMP-9
Gene Fullname matrix metallopeptidase 9
Gene id 4318
Alias Symbols Matrix metalloproteinase-9, MMP9, CLG4B
Swissprot ID P14780
Protein Name Matrix metalloproteinase-9
Description of Target The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metallop
Kit Principle Genway Biotech MMP-9 ELISA Kit (Human) (GWB-ZZD204) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for MMP-9 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, A20-D707) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP-9 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human MMP-9 captured in well.
Kit Component
ComponentAmount
Lyophilized recombinant human MMP-7 standard10 ng/tube x 2
Anti-human MMP-7 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- human MMP-7 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Protein Accession Num NP_004985.2
Nucleotide Accession Num NM_004994.2
Immunogen NSO, A20-D707
Homology No detectable cross-reactivity with any other cytokine.
Standard Range 156pg/ml-10,000pg/ml. Test has a sensitivity of < 5 pg/ml
Datasheets / Downloads GWB-ZZD204 Datasheet
Specimen/Volumes Refer to Manual
Swiss Prot Number P14780
Specificity There is no detectable cross-reactivity with other relevant proteins.
Concentration Lot Specific
Applications ELISA
GI Number 4318
Stability Four months at 4C and eight months at -20C.
Reconstitution and Storage Store at 4C for frequent use, at  -20C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Reactivity Reactive to Human. There is no detectable cross-reactivity with other relevant proteins.
Kit Size 1x96 well plate
Kit Duration ~ 3 Hours
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Intended Use Research Use Only
Key Reference 1. Collier, I. E.; Bruns, G. A. P.; Goldberg, G. I.; Gerhard, D. S. On the structure and chromosome location of the 72-
and 92-kDa human type IV collagenase genes. Genomics 9: 429-434, 1991.
2. Turner, H. E.; Nagy, Z.; Esiri, M. M.; Harris, A. L.; Wass, J. A. H. Role of matrix metalloproteinase 9 in pituitary tumor
behavior. J. Clin. Endocr. Metab. 85: 2931-2935, 2000.
3. Zhang, B.; Ye, S.; Herrmann, S.-M.; Eriksson, P.; de Maat, M.; Evans, A.; Arveiler, D.; Luc, G.; Cambien, F.;
Hamsten, A.; Watkins, H.; Henney, A. M. Functional polymorphism in the regulatory region of gelatinase B gene in
relation to severity of coronary atherosclerosis. Circulation 99: 1788-1794, 1999.
4. Coussens, L. M.; Tinkle, C. L.; Hanahan, D.; Werb, Z. MMP-9 supplied by bone marrow-derived cells contributes to
skin carcinogenesis. Cell 103: 481-490, 2000.
5. Minematsu, N.; Nakamura, H.; Tateno, H.; Nakajima, T.; Yamaguchi, K. Genetic polymorphism in matrix
metalloproteinase-9 and pulmonary emphysema. Biochem. Biophys. Res. Commun. 289: 116-119, 2001.
Additional Information Range: 156pg/ml-10,000pg/ml
:: Principle: Aviva’s human MMP-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human MMP-9 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-9 amount of sample captured in plate.
:: Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion.2 Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture.3 MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.4 The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.5 The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.