Measles Virus IgG



96 Wells
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Product Name Measles Virus IgG - ELISA.
# of Samples 1 x 96 Assays.
Intended Use The Measles virus IgG-ELISA is intended for the qualitative determination of IgG class antibodies against Measles virus in human serum or plasma (citrate).
Introduction Measles or morbilli virus belongs to the RNA viruses of the family Paramyxoviridae. The virions are spherical particles of 150-250 nm in diameter consisting of the ribonucleoprotein with helical symmetry and an envelope with spikes containing the strain-specific and hemagglutinating antigens. Morbilli viruses have no neuraminidase activity. Measles is a classic childhood disease. The virus is endemic: at the age of 20 about 90% of the population has had immunological experience with it. Newborns are protected by maternal antibodies for the first 3-4 months of life; the active disease leaves lifelong immunity. The measles virus has a contagiousity index of about 96%, is worldwide distributed, and can be serious. Bacterial superinfection was a serious threat in the preantibiotic era, but the prognosis of uncomplicated measles is now good. CNS complications such as encephalomyelitis (0.1%) which may occur after the acute phase of measles infection subsides, however still have a high mortality (10%). Prognosis of recovery in these patients is poor. Between 10-30% of all cases are fatal; 20-50% develop significant damages. Subacute sclerosing panencephalitis (SSPE) is a rare (1:1000) degenerative disease of the CNS which is thought to be a slow virus infection.
The presence of virus resp. infection may be identified by
Complement fixation, Hemagglutination-Inhibition, Neutralization
Detection of antibody production by ELISA.
Principles of the assay The qualitative immunoenzymatic determination of IgG-class antibodies against Measles is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Measles antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured Measles-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Measles specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Storage and Stability The reagents are stable up to the expiry date stated on the label when stored at 2-8 °C.
Limitations of the Test Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history, symptomatology as well as serological data.
In immunocompromised patients and newborns serological data only have restricted value.
References Bellini. W.J.,J.S. Rota, P.A. Rota: Virology of Measles Virus.J.Inf.Dis.170 1(1994)5-23 Condorelli, F.,T. Ziegler: Dot immunobinding assay for simultaneous detection of specific immunoglobin G antibodies to measles virus, mumps virus and rubella vrius.J. Clin. Microbiol. (1993) 717-719Doerr, H.W.,St. Geiger: Otimierung der quantitativen Antikörpermessung mit dem ELISA unter Berücksichtigung der klinischen Plausibilität.Lab. Med. 12 (1988) 142-146Dopatka,H.D.,B.Giesendorf:single point quantification of antibody by ELISA without need of a refernce curve.J.of Clin.Lab.Analysis 6(1992) 417-422 Gerike,E.A.Tischer:erfahrungen mit Schutzimpfung gegen Masern, Mumps und Röteln in den neuen Bundesländern.Gesundh.-Wes.(1993) 38-39.

Additional Information

Name Measles Virus IgG
Related Product Names Measles Virus IgG - ELISA
Molecular Weight 0.5
Datasheets / Downloads Click here to download product manual.
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.
Intended Use Research Use Only