MMP3 Human

GWB-284663

10-663-45309

$0.00

Description

Introduction: MMP-3 enzyme is also known as Stromelysin-1or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk . MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.
MMP-3 Human Recombinant produced in HEK293 cells is a proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. 
MMP-3 is purified by proprietary chromatographic techniques.

Biological Activity: The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 150 pmoles/min/µg.

NCBI: AB043995.1

GI: 8176609

Swiss Prot: P08254

Additional Information

Name MMP3 Human
Related Product Names Stromelysin-1, EC 3.4.24.17, Matrix metalloproteinase-3, MMP-3, Transin-1, SL-1, STMY, STR1, STMY1, MGC126102, MGC126103, MGC126104
Gene Symbol MMP 3
Gene id 4314
Alias Symbols Stromelysin-1, EC 3.4.24.17, Matrix metalloproteinase-3, MMP-3, Transin-1, SL-1, STMY, STR1, STMY1, MGC126102, MGC126103, MGC126104, MMP 3 Human, Matrix Metalloproteinase-3 Human Recombinant
Swissprot ID P08254
Protein Name Stromelysin-1
Description of Target Recombinant Human Matrix Metalloproteinase-3
Protein Accession Num NP_002413.1
Host E. Coli
NCBI Acc Number AB043995.1
Product Format The protein was lyophilized from a concentrated (1 mg/ml) solution with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Concentration Lot Specific
Purity Greater than 98% as determined by both(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source HEK293 cells
GI Number 8176609
Format 0.2 um filtered solution in Tris, NaCl and Brij35. Sterile filtered colorless solution
Reconstitution and Storage Lyophilized Matrix Metalloproteinase-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution MMP-3 should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles.
Storage up to 3 weeks at 4C. Store frozen at -20 for long term. Avoid repeat freeze/thaw.
Datasheets/Manuals Printable datasheet for GWB-284663
Intended Use Research Use Only
Additional Information Solubility: It is recommended to reconstitute the lyophilized Stromelysin-1 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
:: Product Introduction: MMP-3 enzyme is also known as Stromelysin-1or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk. MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.