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Mouse IgA ELISA Kit

GWB-1B12B0

40-374-130044

96 Wells
$305.00
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Additional Information

Name Mouse IgA ELISA Kit
Related Product Names Mouse IgA ELISA Kit; N/A Mouse IgAIGHA1
Kit Range 12.5 ng/ml - 400 ng/ml
Sensitivity 0.917 ng/ml
Sample Type Serum, plasma and other biological fluids
Alias Symbols IGHA1, IGHA2, IgA, Ig alpha 1 chain C region, Ig alpha 2 chain C region
Swissprot ID P01878
Protein Name Ig alpha chain C region
Description of Target Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
Kit Principle Genway Biotech IgA ELISA Kit (Mouse) (GWB-1B12B0) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Ig alpha chain C region has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Ig alpha chain C region - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ig alpha chain C region captured in the well.
Kit Component
ComponentAmount
Anti-Mouse IgA Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Mouse IgA Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Mouse
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Applications ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-IgA conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-MOUSE IgA ELISA MICRO PLATE
Anti-Mouse IgA coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. MOUSE IgA CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Mouse
Kit Size 96 well
Kit Duration 100 Minutes
Storage Refer to kit instructions for component storage
Datasheets/Manuals Printable datasheet for GWB-1B12B0
Intended Use Research Use Only
Additional Information Intended Use: The total Mouse IgA test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in biological samples of Mice.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Mouse IgA antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-MOUSE IgA ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse IgA.
7. MOUSE IgA CALIBRATOR
One vial containing a Mouse IgA calibrator.