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Mumps Virus IgG



96 Wells
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Product Name: Mumps Virus IgG – ELISA

# of Samples: 1 x 96 Assays

Intended Use: The Mumps virus IgG-ELISA is intended for the qualitative determination of IgG class antibodies against Mumps virus in human serum or plasma (citrate).

Introduction: Mumps viruses are RNA viruses of the family Paramyxoviridae. The virions are spherical particles of 150-250 nm in diameter consisting of a ribonucleoprotein with helical symmetry and enveloped by matrix protein and a lipid bilayer which contains two spikeline structures: viral hemagglutinin (H) and viral neuraminidase (N). Mumps virus involves primarily the parotid and related salivary glands; however infection can lead to CNS disease and accumulation of the virus in CSF. Mumps (Epidemic Parotitis) is an acute contagious viral disease mostly occuring in children. Nearly 50% of all infections are subclinical. The highest incidence of clinical manifestations is found in the age group of 4 to 15 years. Secondary infections are rare because of long-lasting immunity.10 to 35 % of mumps cases develop orchitis which occurs nearly always after puberty. The process is mostly unilateral and the prognosis usually good. Mumps virus has been one of the most important causes of viral CNS disease (meningitis and encephalitis) in USA; vaccine administration has greatly reduced its incidence.
The presence of virus resp. infection may be identified by:
Complement fixation, Hemadsorption, Hemagglutination-Inhibition
Detection of antibody production by ELISA
Measurement of IgG/IgM antibodies in the serum allows for the determination of the immune status of the patients and recommendations about the necessity of immunization. As prophylaxis live attenuated virus is available and is frequently given in combination with others (measles-mumps-rubella). These vaccinations produce antibody formation in up to 90 % of vaccines.

Principles of the assay: The qualitative immunoenzymatic determination of IgG-class antibodies against Mumps Virus is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiter strip wells are precoated with Mumps Virus antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured Mumps Virus-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Mumps Virus-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.

Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history, symptomatology as well as serological data.
In immunocompromised patients and newborns serological data only have restricted value.

References Berbers, G.A.M et al.: Blocking ELISA for detection of mumps virus antibodies in human sera.J. Virol. Methods 42 (1993) 155-168Berger, R.,M. Just:Corparision of five different tests for mumps antibodies.Infection 8 (1980) 180-183Forsey, T.,M.L. Bentley,P.D. Minor:Mumps vaccines and meningitius.Lancet 340 (1992) 980Friedman,M.,I.Hadari et al.: Virusspecific secretory IgA antibodies as a means of rapid diagnosis of measles and mumps infection.Isr.J.Med.Sci.19 (1993) 881-883Gerike,E.,A. Tischer:Erfahrung mit der Schutzimpfung gegen Masern,Mumps und Röteln in den neuen Bundesländern. Gesundh.-Wes.55 (1993) 38-29

Additional Information

Name Mumps Virus IgG
Related Product Names Mumps Virus IgG – ELISA
Molecular Weight 0.5
Datasheets / Downloads GWB-B62C8F Datasheet
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.
Intended Use Research Use Only