Mouse NGF/NGF beta ELISA Kit

GWB-ZZD031

1x96 well plate
$428.40
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Description

Short Description: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

Additional Information

Name Mouse NGF/NGF beta ELISA Kit
Related Product Names Beta nerve growth factor|Beta NGF|HSAN5|MGC161426|MGC161428|Nerve growth factor (beta polypeptide)|Nerve growth factor beta polypeptide|Nerve growth factor beta subunit|NGFB|NID67
Drywet Surcharge 0
Kit Range 31.2 pg/mL-2,000 pg/mL
Sensitivity < 1 pg/mL
Sample Type cell culture supernates and serum
Kit Detection Colorimetric, OD 450 nm
Gene Symbol NGF
Gene Fullname nerve growth factor
Gene id 18049
Alias Symbols Beta-nerve growth factor, NGF, NGFB
Swissprot ID P01139
Protein Name Beta-nerve growth factor
Description of Target Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensor
Kit Principle Genway Biotech NGF ELISA Kit (Mouse) (GWB-ZZD031) is based on standard sandwich enzyme-linked immune-sorbent assay technology. A rat monoclonal antibody specific for NGF has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, S122-G241) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NGF is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse NGF captured in well.
Kit Component
ComponentAmount
Lyophilized recombinant mouse NGF standard10 ng/tube x 2
Anti-mouse NGF Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- mouse NGF antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Protein Accession Num NP_001106168.1
Nucleotide Accession Num NM_001112698.2
Immunogen NSO, S122-G241
Homology No detectable cross-reactivity with any other cytokine.
Standard Range 31.2pg/ml-2000pg/ml. Test has a sensitivity of < 1 pg/ml
Datasheets / Downloads GWB-ZZD031 Datasheet
Specimen/Volumes Refer to Manual
Swiss Prot Number P01139
Specificity There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.
Concentration Lot Specific
Applications ELISA
GI Number 18049
Stability Four months at 4C and eight months at -20C.
Reconstitution and Storage Store at 4C for frequent use, at  -20C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Reactivity Reactive to Mouse. There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.
Kit Size 1x96 well plate
Kit Duration ~ 3 Hours
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Intended Use Research Use Only
Key Reference 1. Ullrich, A.; Gray, A.; Berman, C.; Dull, T. J. Human beta-nerve growth factor gene sequence highly homologous to
that of mouse. Nature 303: 821-825, 1983.
2. Sanico, A. M.; Stanisz, A. M.; Gleeson, T. D.; Bora, S.; Proud, D.; Bienenstock, J.; Koliatsos, V. E.; Togias, A.
Nerve growth factor expression and release in allergic inflammatory disease of the upper airways. Am. J. Resp. Crit.
Care Med. 161: 1631-1635, 2000.
Additional Information Range: 31.2pg/ml-2000pg/ml
:: Principle: Aviva’s mouse NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse NGF specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse NGF amount of sample captured in plate.
:: Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.