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Rat BDNF ELISA Kit

GWB-ZZD023

1x96 well plate
$385.56
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Description

Short Description: Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.

Additional Information

Name Rat BDNF ELISA Kit
Related Product Names Abrineurin|ANON2|Brain derived neurotrophic factor|BULN2|MGC34632|Neurotrophin
Drywet Surcharge 0
Gene Symbol BDNF
Gene Fullname brain-derived neurotrophic factor
Gene id 24225
Alias Symbols brain-derived neurotrophic factor, BDNF, ANON2, BULN2
Protein Name Brain-derived neurotrophic factor
Description of Target BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult Rat brain, suggesting that these proteins are involved in the maintenance of the adu
Swissprot ID P23363
Protein Accession Num NP_001257559.1
Nucleotide Accession Num NM_001270630.1
Species Reactivity Rat
Sensitivity < 15 pg/ml
Kit Range 31.2 pg/ml-2,000 pg/ml
Immunogen sf21, H129-R247
Homology No detectable cross-reactivity with any other cytokine.
Standard Range 31.2pg/ml-2000pg/ml. Test has a sensitivity of < 2 pg/ml
Sample Type cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Datasheets / Downloads GWB-ZZD023 Datasheet
Specimen/Volumes Refer to Manual
Swiss Prot Number P23363
Specificity There is no detectable cross-reactivity with other relevant proteins.
Concentration Lot Specific
Applications ELISA
GI Number 24225
Stability Four months at 4C and eight months at -20C.
Reconstitution and Storage Store at 4C for frequent use, at -20C for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Reactivity Reactive to Rat. There is no detectable cross-reactivity with other relevant proteins.
Kit Size 1x96 well plate
Kit Principle Genway Biotech BDNF ELISA Kit (Rat) (GWB-ZZD023) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for BDNF has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (sf21, H129-R247) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BDNF is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Rat BDNF captured in well.
Kit Component
ComponentAmount
Lyophilized recombinant rat BDNF standard10 ng/tube x 2
Anti-rat BDNF Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- rat BDNF antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Kit Detection Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Intended Use Research Use Only
Key Reference 1. Jones, K. R.; Reichardt, L. F. Molecular cloning of a human gene that is a member of the nerve growth factor family.
Proc. Nat. Acad. Sci. 87: 8060-8064, 1990.
2. Kovalchuk, Y.; Hanse, E.; Kafitz, K. W.; Konnerth, A. Postsynaptic induction of BDNF-mediated long-term
potentiation. Science 295: 1729-1734, 2002.
3. Lyons, W. E.; Mamounas, L. A.; Ricaurte, G. A; Coppola, V.; Reid, S. W.; Bora, S. H.; Wihler, C.; Koliatsos, V. E.;
Tessarollo, L. Brain-derived neurotrophic factor-deficient mice develop aggressiveness and hyperphagia in
conjunction with brain serotonergic abnormalities. Proc. Nat. Acad. Sci. 96: 15239-15244, 1999.
4. Rios, M.; Fan, G.; Fekete, C.; Kelly, J.; Bates, B.; Kuehn, R.; Lechan, R. M.; Jaenisch, R. Conditional deletion of
brain-derived neurotrophic factor in the postnatal brain leads to obesity and hyperactivity. Molec. Endocr. 15:
1748-1757, 2001.
5. Baker-Herman, T. L.; Fuller, D. D.; Bavis, R. W.; Zabka, A. G.; Golder, F. J.; Doperalski, N. J.; Johnson, R. A.;
Watters, J. J.; Mitchell, G. S. BDNF is necessary and sufficient for spinal respiratory plasticity following intermittent
hypoxia. Nature Neurosci. 7: 48-55, 2004.
Additional Information Range: 31.2pg/ml-2000pg/ml
:: Principle: Aviva’s rat BDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat BDNF specific-specific polyclonal antibodies were precoated onto 96-well plates. The rat specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BDNF amount of sample captured in plate.
:: Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically.2 BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system.3 BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state.4 It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.5