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C3 ELISA Kit (Human) (GWB-EKA007)

GWB-EKA007

1x96 well plate
$400.00
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Additional Information

Name C3 ELISA Kit (Human) (GWB-EKA007)
Description of Target Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
Kit Range 3.9 - 500 ng/mL
Sensitivity <3.0 ng/mL
Sample Type Serum, plasma, and other biological fluids
Assay Time ~2.5 hours
Kit Detection Colorimetric, OD 450 nm
Specificity/Cross-Reactivity
Species Reactivity
Species Cross Reactivity (%)
Chicken 0%
Dog 0%
Mouse 5%
Pig 5%
Rat 5%


Cross-Reactivity
Antigen Cross Reactivity (%)
AAT 11%
APOA1 0%
A2M 0%
Fibrinogen 0%
Haptoglobin 0%
HDL 0%
H. HSA 0%
IgA 0%
IgG 0%
IgM 0%
LDL 0%
Orosomucoid 0%
Transferrin 0%
Gene Symbol C3
Gene Fullname Complement C3
Gene id 718
Alias Symbols C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1
Swissprot ID P01024
Species Human
Protein Name Complement C3
Kit Principle GenWay Biotech C3 ELISA Kit (Human) (GWB-EKA007) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C3 has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for C3is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C3 captured in well.
Kit Component
ComponentQuantity
C3 Microplate1 x 96 wells plate
C3 Lyophilized Standard2 x vials (500 ng/vial)
C3 HRP-Detection Antibody1 x 10 µL
Assay Diluent B1 x 30 mL
20X Wash Buffer1 x 30 mL
TMB Substrate1 x 12 mL
Stop Solution1 x 12 mL
Datasheets/Manuals Printable datasheet for GWB-EKA007
Dilution Assay Diluent
Species Reactivity Mouse, Pig, Rat