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IgA ELISA Kit (Human) (GWB-EKA008)


1x96 well plate
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Additional Information

Name IgA ELISA Kit (Human) (GWB-EKA008)
Description of Target Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed:2241915).
Kit Range 1.95 – 250 ng/mL
Sensitivity ≤ 1.0 ng/mL
Sample Type Serum, plasma, and other biological fluids
Assay Time ~2.5 hours
Kit Detection Colorimetric, OD 450 nm
Species Reactivity
SpeciesCross Reactivity (%)

AntigenCross Reactivity (%)
H. HSA0%
Gene Symbol IGA
Gene Fullname Immunoglobulin heavy constant alpha 1 and 2
Gene id 3493
Alias Symbols Ig alpha-1 chain C region, MU; VH; AGM1
Swissprot ID P01876
Species Human
Protein Name Immunoglobulin heavy constant alpha 1
Kit Principle GenWay Biotech IGA ELISA Kit (Human) (GWB-EKA008) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGA has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for IGAis added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGA captured in well.
Kit Component
Anti-IgA Microplate1 x 96 wells plate
IgA Lyophilized Standard2 x vials (250 ng/vial)
IgA HRP-Detection Antibody1 x 10 µL
Assay Diluent A1 x 30 mL
20X Wash Buffer1 x 30 mL
TMB Substrate1 x 12 mL
Stop Solution1 x 12 mL
Datasheets/Manuals Printable manual for GWB-EKA008
Dilution Assay Diluent