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SERPINA1 ELISA Kit (Human) (GWB-EKA005)

GWB-EKA005

1x96 well plate
$400.00
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Additional Information

Name SERPINA1 ELISA Kit (Human) (GWB-EKA005)
Description of Target The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Kit Range 0.98 - 500 ng/ml
Sensitivity < 0.6 ng/mL
Sample Type Serum, plasma, and other biological fluids
Assay Time ~2.5 hrs
Kit Detection Colorimetric, OD 450 nm
Specificity/Cross-Reactivity
Species Reactivity
Species Cross Reactivity (%)
Chicken 2%
Dog 0%
Mouse 0%
Pig 0%
Rat 0%


Cross-Reactivity
Antigen Cross Reactivity (%)
APO 0%
A2 0%
C3 0%
Fibrinogen 0%
Haptoglobin 8%
HDL 0%
H. HSA 0%
IgA 0%
IgG 0%
IgM 0%
LDL 0%
Orosomucoid 6%
Transferrin 0%
Gene Symbol SERPINA1
Gene Fullname Alpha-1-antitrypsin
Gene id 5265
Alias Symbols Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1, AAT, PI, PRO0684, PRO2209
Swissprot ID P01009
Species Human
Protein Name Alpha-1-antitrypsin
Kit Principle GenWay Biotech SERPINA1 ELISA Kit (Human) (GWB-EKA005) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINA1 has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for SERPINA1is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINA1 captured in well.
Kit Component
ComponentQuantity
Anti-SERPINA1 Microplate96 Wells (12 x 8 Well strips)
SERPINA1 Lyophilized Standard2 vials
SERPINA1 HRP-Detection Anitbody1 x 10 µL
Assay Diluent1 x 30 mL
20X Wash Buffer1 x 30 mL
TMB Substrate1 x 12 mL
Stop Solution1 x 12 mL
Datasheets/Manuals Printable manual for GWB-EKA005
Dilution 1X PBS
Species Reactivity Chicken, Dog