Transferrin ELISA Kit (Human) (GWB-EKA012)


1x96 well plate
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Additional Information

Name Transferrin ELISA Kit (Human) (GWB-EKA012)
Description of Target Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Kit Range 6.25 - 400 ng/ml
Sensitivity < 3.2 ng/ml
Sample Type Serum, plasma and other biological fluids
Assay Time ~2.5 hrs
Kit Detection Colorimetric, OD 450 nm
Species Reactivity
Species Cross Reactivity (%)
Chicken 0%
Dog 0%
Mouse 0%
Pig 0%
Rat 0%

Antigen Cross Reactivity (%)
AAT 0%
APO 0%
A2 0%
C3 0%
Fibrinogen 0%
Haptoglobin 0%
HDL 0%
H. HSA 0%
IgA 0%
IgG 0%
IgM 0%
LDL 0%
Orosomucoid 0%
Gene Symbol TF
Gene Fullname Serotransferrin
Gene id 7018
Alias Symbols Transferrin, Beta-1 metal-binding globulin, Siderophilin
Swissprot ID P02787
Species Human
Protein Name Serotransferrin
Kit Principle GenWay Biotech Transferrin ELISA Kit (Human) (GWB-EKA012) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Transferrin has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for Transferrin added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Transferrin captured in well.
Kit Component
Anti-Transferrin Microplate96 Wells (12 x 8 Well strips)
Transferrin Lyophilized Standard2 vials
Transferrin HRP-Detection Anitbody1 x 10 µL
Assay Diluent1 x 30 mL
20X Wash Buffer1 x 30 mL
TMB Substrate1 x 12 mL
Stop Solution1 x 12 mL
Datasheets/Manuals Printable manual for GWB-EKA012
Dilution 1X PBS