Rat Fibronectin ELISA Kit

GWB-16BC00

40-374-130068

96 Wells
$435.00
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Additional Information

Name Rat Fibronectin ELISA Kit
Related Product Names Rat Fibronectin ELISA Kit
Drywet Surcharge 0
Kit Range 6.25 ng/ml - 200 ng/ml
Sensitivity 2.697 ng/ml
Sample Type Serum, plasma and other biological fluids
Alias Symbols Fibronectin 1, FN1, FN
Swissprot ID P04937
Protein Name Fibronectin
Description of Target Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compac
Kit Principle Genway Biotech Fibronectin ELISA Kit (Rat) (GWB-16BC00) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Fibronectin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Fibronectin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fibronectin captured in the well.
Kit Component
ComponentAmount
Anti-Rat Fibronectin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat Fibronectin Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Rat
Standard Range Refer To Manual
Product Format In Solution
Datasheets / Downloads GWB-16BC00 Datasheet
Specimen/Volumes Refer To Manual
Applications ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-FCT conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-RAT FIBRONECTIN ELISA MICRO PLATE
Anti-Rat FCT coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. RAT FIBRONECTIN CALIBRATOR
The lyophilized Rat Fibronectin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Rat
Kit Size 96 well
Kit Duration 100 Minutes
Storage Refer to kit instructions for component storage
Intended Use Research Use Only
Additional Information Intended Use: The Fibronectin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibronectin in biological samples of rat.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat Fibronectin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT FIBRONECTIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat Fibronectin.
7. RAT FIBRONECTIN CALIBRATOR
One vial containing a lyophilized Rat Fibronectin calibrator.