Genway's office will be closed for Good Friday - April 10, 2020.

Human Alpha-1-Antitrypsin ELISA Quantitation Kit (GWB-1F2730)



10x96 Wells


Species Human
Sample Type Serum or plasma. For the quantitative determination of human Alpha 1-Antitrypsin levels in serum or other biological samples. Blood, Connective Tissue Diseases, Lung Diseases, Liver, Pathologic Processes.
Product Name Human alpha 1-Antitrypsin.
Sample Type Serum or plasma.
# of Samples 10X96 wells.
Storage and Stability -20C.
Range of Detection 1.4-90 ng/ml
Shelf life one year from date of receipt.
Assay Condition The kit performance has been optimized for the protocol and materials listed below using standard dilutions of human Alpha-1-Antitrypsin in the 1.4-90 ng/ml range. The operator must determineappropriate dilutions of reagents for alternative assay conditions. ELISA assay reactivity is sensitive tovariations in operator, pipetting and washing techniques, incubation time, temperature, composition of reagents, and other experimental variables. Assay optimization may be required to generate the standardcurve and fit the samples in the specified detection range.
Country of Origin United States of America.
Assay Use For Research Use Only (RUO). Not for diagnostic or therapeutic use in humans oranimals. Not for animal or human consumption.
Protocols from Publications Citation: 1: Pott GB, Chan ED, Dinarello CA, Shapiro L. Alpha-1-antitrypsin is an endogenous inhibitor of proinflammatory cytokine production in whole blood. J Leukoc Biol. 2009 May;85(5):886-95. Epub 2009 Feb 5. PubMed PMID: 19197072; PubMed Central PMCID: PMC2669404.
Experiment Name ELISA.
Experiment Background
  1. Whole blood collection from AAT-deficient patients.
  2. AAT-deficient patients were diagnosed with chronic obstructive pulmonary disease and treated with inhaled fluticasone-salmeterol, and eight of the nine patients were treated with inhaled albuterol. Other prescribed medications included fluoxetine, tiotropium, iprotropium, lansoprazole, atorvastatin, alendronate, and fexofenadine.
  3. Human alpha 1-Antitrypsin (AAT) is the most abundant serine protease inhibitor in blood, and AAT possesses anti-inflammatory activity in vitro and in vivo. Here, Gregory et. al. showed that in vitro stimulation of whole blood from persons with a genetic AAT deficiency resulted in enhanced cytokine production compared with blood from healthy subjects.
Experiment Steps Determine AAT concentrations from AAT-deficient and healthy volunteers in heparanized plasma using a human AAT quantitative ELISA.
Function Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Subcellular Location Secreted.
Tissue Specificity Plasma.
Domain The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Several isomers are observed, resulting from the combination of different N-linked glycan structures and mature N-terminus. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary whereas glycan at Asn-70 is di-antennary with trace amounts of tri-antennary, and glycan at Asn-271 is exclusively di-antennary. The structure of the antennas is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennas are fucosylated, which forms a Lewis-X determinant.
  • Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
Polymorphism The sequence shown is that of the M1V allele which is the most common form of PI (44 to 49%). Other frequent alleles are: M1A 20 to 23%; M2 10 to 11%; M3 14 to 19%.
  • The major physiological function of AAT is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). A hereditary deficiency of AAT, is associated with a 20-30 fold increased risk of developing chronic obstructive pulmonary disease.
  • Deficiency of the normal inhibitor in individuals homozygous for allele Z or M-Malton can result in the development of chronic emphysema or infantile liver cirrhosis.
  • Variant Pittsburgh is the cause of bleeding diathesis.
Miscellaneous The aberrant form is found in the plasma of chronic smokers, and persists after smoking is ceased. It can still be found ten years after smoking has ceased.
Similarity Belongs to the serpin family.
Product Protocol To see this product used in a peer reviewed journal, click the following link α-1-Antitrypsin is an endogenous inhibitor of proinflammatory cytokine production in whole blood.

Additional Information

Name Human Alpha-1-Antitrypsin ELISA Quantitation Kit (GWB-1F2730)
Related Product Names Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Human alpha 1-Antitrypsin ELISA Kit; Alpha-1 protease inhibitor, Alpha-1-antiproteinase, AAT, PI Human alpha 1 Antitrypsin, SERPINA1, PI, AAT ELISA, A1AT
Short Description Human alpha 1-Antitrypsin, AAT, AAT ELISA
Swissprot ID P01009
NCBI Acc Number NP_000286.3
Molecular Weight 46737
Datasheets/Manuals Printable datasheet for GWB-1F2730
Intended Use Research Use Only