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Yersinia IgA ELISA



1x96 well plate
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Enzyme immunoassay based on microtiter platefor the detection and quantitative determinationof human IgA antibodies against Yersinia enterocoliticain serum and plasma

Intended Use
The GenWay Yersinia IgA Antibody ELISA Test Kit has been designed for the the detection andthe quantitative determination of specific IgA antibodies against Yersinia enterocolitica in serumand plasma. Further applications in other body fluids are possible and can be requested from theTechnical Service of GenWay.This assay is intended for research use only.

General Information
The pathogenic germs Yersinia pestis, Y. pseudotuberculosis, Y. enterocolitica and Y. ruckeribelong to the genus Yersinia as a member of the enterobacteriacea family. All the species ofmedical importance occur facultatively inside the cells, which leads to the characteristicalinflammation of the lymphatic tissue in the course of an illness. Yersinia enterocolitica is taken uporally, and the symptoms in an individual are terminal ileitis as well as diarrhoea. It is difficult tomake a separation from appendicitis by differential diagnosis. In the course of a retardedimmunological reaction, extraintestinal manifestations like erythema nodosum, uveitis, and arthritiscan appear. It has been claimed that the background for a reactive arthritis caused by Yersiniaconsists in the local synthesis of antibodies in the joints (synovial fluid).Yersinia can be characterized without problems by standardized bacterial tests, after isolation in apure culture. Following a human disease, above all the serotypes O3, O8 and O9 are found. Crossreactivitiesto Brucella are described and have to be taken into account by the differential diagnosis.The classical serological detection method has always been the Widal test. Further increasinglypopular methods are the HAT, the KBR test as well as the ELISA. The enzyme immunoassay ischaracterized on the one hand side by a high sensitivity, but also by the possibility to differentiatebetween IgG and IgA/IgM antibodies. The immunoglobulin classes IgA and IgM should be referredto as a criterium of interpretation for an active process, when an arthritic disease is suspected.Because there exists also an immunological similarity with the thyrotropin receptor, the test canalso be employed for the confirmation of the autoimmune Graves’ disease. As a confirmatory testwith the possibility of an identification of separate bacterial proteins as well as for the exclusion ofcross-reactivities, the Western Blot method has proved successful.

Principle of the Test
The GenWay Yersinia enterocolitica IgA antibody test kit is based on the principle of the enzymeimmunoassay (EIA). Yersinia antigen is bound on the surface of the microtiter strips. Dilutedsample serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A bindingbetween the IgA antibodies of the serum and the immobilized Yersinia antigen takes place. After aone hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order toremove unbound material. Then ready-to-use anti-human-IgA peroxidase conjugate is added andincubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted andincubated for 20 minutes, inducing the development of a blue dye in the wells. The colordevelopment is terminated by the addition of a stop solution, which changes the color from blue toyellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. Theconcentration of the IgA antibodies is directly proportional to the intensity of the color.

1. Bottone, E.J. (ed.). Yersinia enterocolitica. CRC Press, Boca Raton (1981).
2. Brubaker, R.R. The genus Yersinia: biochemistry and genetics. Curr. Top. Microbiol., 57: 111(1972).
3. Butler, T. Plague and other Yersinia infections. Plenum Publishing Corp., New York (1983).
4. Heesemann, J. et al. Experimental Yersinia enterocolitica infections; a model for humanyersiniosis. APMIS, 101: 417 (1993).
5. Kittelberger, R. et al. Serological cross-reactivity between Brucella abortus and Yersiniaenterocolitica. Vet. Microbiol., 47: 271 (1995).
6. Luo, G. et al. Purification and characterization of Yersinia enterocolitica proteins. J. Immunol.,152: 2555 (1994).
7. Möaki-Ikola, O. et al. Yersinia-specific antibodies in serum and synovial fluid. Ann. Rheum.Dis., 53: 535 (1994).

Additional Information

Name Yersinia IgA ELISA
Related Product Names Yersinia IgA ELISA
Datasheets/Manuals Printable manual for GWB-18D386
Intended Use Research Use Only