Yersinia IgG ELISA



1x96 well plate
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Enzyme immunoassay based on microtiter plate for the detection and quantitative determination of human IgG antibodies against Yersinia enterocoliticain serum and plasma.
Intended Use
The GenWay Yersinia IgG Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgG antibodies against Yersinia enterocolitica in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of GenWay. This assay is intended for research use only.
General Information The pathogenic germs Yersinia pestis, Y. pseudotuberculosis, Y. enterocolitica and Y. ruckeri belong to the genus Yersinia as a member of the enterobacteriacea family. All the species of medical importance occur facultatively inside the cells, which leads to the characteristical inflammation of the lymphatic tissue in the course of an illness. Yersinia enterocolitica is taken up orally, and the symptoms in an individual are terminal ileitis as well as diarrhoea. It is difficult to make a separation from appendicitis by differential diagnosis. In the course of a retarded immunological reaction, extraintestinal manifestations like erythema nodosum, uveitis, and arthritis can appear. It has been claimed that the background for a reactive arthritis caused by Yersinia consists in the local synthesis of antibodies in the joints (synovial fluid). Yersinia can be characterized without problems by standardized bacterial tests, after isolation in a pure culture. Following a human disease, above all the serotypes O3, O8 and O9 are found. Crossreactivities to Brucella are described and have to be taken into account by the differential diagnosis. The classical serological detection method has always been the Widal test. Further increasingly popular methods are the HAT, the KBR test as well as the ELISA. The enzyme immunoassay is characterized on the one hand side by a high sensitivity, but also by the possibility to differentiate between IgG and IgA/IgM antibodies. The immunoglobulin classes IgA and IgM should be referred to as a criterium of interpretation for an active process, when an arthritic disease is suspected. Because there exists also an immunological similarity with the thyrotropin receptor, the test can also be employed for the confirmation of the autoimmune Graves’ disease. As a confirmatory test with the possibility of an identification of separate bacterial proteins as well as for the exclusion of cross-reactivities, the Western Blot method has proved successful.
Principle of the Test The GenWay Yersinia enterocolitica IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Yersinia antigen is bound on the surface of the microtiter strips. Diluted sample serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Yersinia antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.

Additional Information

Name Yersinia IgG ELISA
Related Product Names Yersinia IgG ELISA
Datasheets/Manuals Printable manual for GWB-1B9B45
Intended Use Research Use Only