Eml2 ELISA Kit (Rat) (GWB-KBBYE7)

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GWB-KBBYE7

96 Wells
$799.00
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Additional Information

Name Eml2 ELISA Kit (Rat) (GWB-KBBYE7)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.126 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol EML2
Gene id 192360
Alias Symbols Echinoderm microtubule-associated protein-like 2, Emap, Emap2, Emap-2, EMAP-2, Eml1
Swissprot ID Q6P6T4
Protein Name echinoderm microtubule-associated protein-like 2
Description of Target Tubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.
Kit Principle Genway Biotech Eml2 ELISA Kit (Rat) (GWB-KBBYE7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Eml2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Eml2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Eml2 captured in the well.
Kit Component
ComponentAmount
Anti-RBM24 Microplate96 Wells (12 x 8 Well Strips)
RBM24 Lyophilized Standard2 x 20 ng
100X Biotinylated RBM24 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001180197
Nucleotide Accession Num NM_001193268
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBYE7
Species Reactivity Rat