Eng ELISA Kit (Rat) (GWB-KBBZG5)

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GWB-KBBZG5

96 Wells
$649.00
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Additional Information

Name Eng ELISA Kit (Rat) (GWB-KBBZG5)
Kit Range 0.781 - 50 ng/mL
Sensitivity < 0.35 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ENG
Gene id 497010
Alias Symbols CD105, END, HHT1, ORW, ORW1, Osler-Rendu-Weber Syndrome 1
Swissprot ID Q6Q3E8
Protein Name Uncharacterized protein
Kit Principle Genway Biotech Eng ELISA Kit (Rat) (GWB-KBBZG5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Eng has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Eng is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Eng captured in the well.
Kit Component
ComponentAmount
Anti-Cygb Microplate96 Wells (12 x 8 Well Strips)
Cygb Lyophilized Standard2 x 50 ng
100X Biotinylated Cygb Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001010968
Nucleotide Accession Num NM_001010968
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)98-105%99-105%98-105%94-105%
EDTA Plasma (n=5)80-98%91-104%82-94%92-101%
Heparin Plasma (n=5)90-103%86-104%78-93%84-99%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)81-9086
EDTA Plasma (n=5)81-9385
Heparin Plasma (n=5)97-104101
Datasheets/Manuals Printable datasheet for GWB-KBBZG5
Species Reactivity Rat