Eno2 ELISA Kit (Mouse) (GWB-KBBRL4)

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GWB-KBBRL4

96 Wells
$619.00
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Additional Information

Name Eno2 ELISA Kit (Mouse) (GWB-KBBRL4)
Kit Range 78.12 - 5,000 pg/mL
Sensitivity < 32 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ENO2
Gene id 13807
Alias Symbols ENO2, Enolase 2, Gamma Enolase, 2-phospho-D-glycerate hydro-lyase, Neural enolase
Swissprot ID Q545V3
Protein Name gamma-enolase
Kit Principle Genway Biotech Eno2 ELISA Kit (Mouse) (GWB-KBBRL4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Eno2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Eno2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Eno2 captured in the well.
Kit Component
ComponentAmount
Anti-Il10ra Microplate96 Wells (12 x 8 Well Strips)
Il10ra Lyophilized Standard2 x 5 ng
100X Biotinylated Il10ra Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_038537
Nucleotide Accession Num NM_013509
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-98%81-89%81-94%84-93%
EDTA Plasma (n=5)98-105%87-103%97-105%87-97%
Heparin Plasma (n=5)79-101%87-101%78-91%79-96%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)82-10287
EDTA Plasma (n=5)92-105101
Heparin Plasma (n=5)80-9680
Datasheets/Manuals Printable datasheet for GWB-KBBRL4
Species Reactivity Mouse