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In observance of Memorial Day, Genway Biotech office will be closed on Monday, May 28th, 2018

ACTH ELISA

GWB-D2B7F8

40-101-325001

1x96 well plate
$701.60
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Description

Product Name: ACTH ELISA

Sample Type: Plasma

# of Samples: 1x96 well

Intended Use: The GenWay, Inc. ACTH ELISA Kit is intended for the quantitative determination of ACTH Adrenocorticotropic Hormone) in human plasma

Principle of the test: The GenWay ACTH Immunoassay is a two-site ELISA for the measurement of the biologically active 39 amino acid chain of ACTH. One antibody is prepared to bind only the C-terminal ACTH 34-39 and this antibody is biotinylated. The other antibody is prepared to bind only the mid-region and N-terminal ACTH 1-24 and this antibody is labeled with HRP for detection. In this assay, calibrators, controls, or patient samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidincoated microplate well. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the TMB substrate. Stop solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of ACTH in the sample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of ACTH present in the controls and patient samples are determined directly from this curve.

Summary and Explanation of Test: Storage and Stability: 1. Store the kit at 2 - 8 C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light.

Storage and Stability: 1. Store the kit at 2 - 8 C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light.

Limitation of the Test: The GenWay ACTH ELISA kit has exhibited no “high dose hook effect” with samples spiked with 20,000 pg/ml of ACTH. Samples with ACTH levels greater than the highest calibrator, however, should be diluted and reassayed for correct values. Like any analyte used as a diagnostic adjunct, ACTH results must be interpreted carefully with the overall clinical presentations and other supportive diagnostic tests.

Product Name: ACTH ELISA

Additional Information

Name ACTH ELISA
Related Product Names ACTH ELISA
Molecular Weight 0.5
Datasheets / Downloads GWB-D2B7F8 Datasheet
Intended Use Research Use Only