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Total Bile Acids Assay (Dual vial liquid stable) (Enzyme cycling method)

GWB-BQK086

BQ 042A-EALD

430 Tests
$325.60
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Description

DescriptionThe Total Bile Acids Enzymatic Assay Kit is intended for the in vitro quantitative determination of serum total bile acids (TBA). Total bile acids are metabolized in the liver and hence, serve as a marker for normal liver function. Serum total bile acids are increased in patients with acute hepatitis, chronic hepatitis, liver sclerosis and liver cancer. The reagents of the assay kit are stable liquid formulation that allows ease of use coupled with enhanced performance characteristics. In the presence of Thio-NAD, the enzyme 3-α.hydroxysteroid dehydrogenase (3-α-HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible and 3-α-HSD can convert 3-keto steroids and Thio-NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 405nm.
SpeciesHuman
Sample TypeSerum or Plasma

Additional Information

Name Total Bile Acids Assay (Dual vial liquid stable) (Enzyme cycling method)
Short Description The Total Bile Acids Enzymatic Assay Kit is intended for the in vitro quantitative determination of serum total bile acids (TBA). Total bile acids are metabolized in the liver and hence, serve as a marker for normal liver function. Serum total bile acids are increased in patients with acute hepatitis, chronic hepatitis, liver sclerosis and liver cancer. The reagents of the assay kit are stable liquid formulation that allows ease of use coupled with enhanced performance characteristics. In the presence of Thio-NAD, the enzyme 3-α.hydroxysteroid dehydrogenase (3-α-HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible and 3-α-HSD can convert 3-keto steroids and Thio-NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 405nm.
Reactivity Human
Datasheets/Manuals Printable manual for GWB-BQK086