Equine CRP ELISA (GWB-374Z28) (Aviva Cat. No. OKIA00038)

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GWB-374Z28

Aviva Cat. No.: OKIA00038

Description

Short Description: Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in humans by Tillet and Frances in 19301. This C-reactive protein (CRP) is so named because it is able to effect precipitation of somatic C-polysaccharide of Streptococcus pneumonia. CRP is an alpha globulin with a mass of 110,000 to 140,000 daltons, and composed of five identical subunits, which are non-covalently assembled as a cyclic pentamer. It is synthesized in the liver and, in humans, is normally present as a trace constituent of serum at levels less that 0.3 mG/dL. The levels in serum rise quickly following acute tissue damage and also falls very rapidly once the stimulus is removed. It has been proposed that the function of CRP is to aid in complement activation, influence phagocytic cell function, and augment cell mediated cytotoxicity. Investigations over the past few years have shown that quantification of these in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in humans, but in companion animals and farm herds as well2.

Additional Information

Name Equine CRP ELISA (GWB-374Z28)
Related Product Names C-Reactive Protein, CRP, PTX1, Horse C-Reactive Protein ELISA, Horse CRP, Equine C-Reactive Protein ELISA Kit
Kit Range 6.25 ng/ml - 200 ng/ml
Sensitivity 1.198 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene id 100057752
Alias Symbols CRP, PTX1
Swissprot ID F6PZG1
Protein Name Pentaxin
Kit Principle Genway Biotech CRP ELISA Kit (Horse) (GWB-374Z28) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Pentaxin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Pentaxin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pentaxin captured in the well.
Kit Component
ComponentAmount
Anti-Horse CRP Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Horse CRP Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Horse
Standard Range 200 ng/ml-6.25 ng/ml
Product Format In Solution
Specimen/Volumes Refer To Manual
Concentration Lot Specific
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-CRP conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HORSE CRP ELISA MICRO PLATE
Anti-Horse CRP coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HORSE CRP CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Equine
Kit Size 96 well
Kit Duration 70 Minutes
Datasheets/Manuals Printable datasheet for GWB-374Z28
Storage Refer to kit instructions for component storage
Species Reactivity Horse
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The CRP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in Horse biological samples.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Horse CRP antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HORSE CRP ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Horse CRP.
7. HORSE CRP CALIBRATOR
One vial containing Horse CRP calibrator.