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96 Wells
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Additional Information

Name ERLIN1 ELISA Kit (Human) (GWB-KBBBV9)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.107 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ERLIN1
Gene id 10613
Alias Symbols C10orf69, Endoplasmic reticulum lipid raft-associated protein 1, Erlin-1, KE04, KEO4, Protein KE04, SPFH1, SPFH domain-containing protein 1, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1
Swissprot ID O75477
Protein Name Erlin-1
Description of Target Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling p
Kit Principle Genway Biotech ERLIN1 ELISA Kit (Human) (GWB-KBBBV9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ERLIN1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ERLIN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ERLIN1 captured in the well.
Kit Component
Anti-ERLIN1 Microplate96 Wells (12 x 8 Well Strips)
ERLIN1 Lyophilized Standard2 x 20 ng
100X Biotinylated ERLIN1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001094096
Nucleotide Accession Num NM_001100626
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBBV9
Species Reactivity Human