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96 Wells

Additional Information

Name ERRa ELISA Kit (Rat) (GWB-KBBHE2)
Kit Range 0.156-10 ng/mL
Sensitivity <0.053ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ERRa
Gene id 293701
Alias Symbols ERRalpha, ERR-alpha, Errra, Estrogen-related receptor alpha, Nr3b1, Nuclear receptor subfamily 3 group B member 1, Steroid hormone receptor ERR1
Swissprot ID Q5QJV7
Protein Name steroid hormone receptor ERR1
Description of Target Estrogen related receptor, alpha [rgd, feb 2006]
Kit Principle Genway Biotech ERRa ELISA Kit (Rat) (GWB-KBBHE2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ERRa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ERRa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ERRa captured in the well.
Kit Component
Anti-ERRa Microplate96 Wells (12 x 8 Well Strips)
ERRa Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ERRa Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001008511.2
Nucleotide Accession Num NM_001008511.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBHE2
Species Reactivity Rat