Esr2 ELISA Kit (Mouse) (GWB-KBBXP1) (Aviva Cat. No. OKCD00798)

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GWB-KBBXP1

Aviva Cat. No.: OKCD00798

Additional Information

Name Esr2 ELISA Kit (Mouse) (GWB-KBBXP1)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.142 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ESR2
Gene id 13983
Alias Symbols ER[b], ERbeta, ER beta, ER-beta, Estrb, Estrogen receptor beta, Nr3a2, Nuclear receptor subfamily 3 group A member 2, oestrogen receptor beta
Swissprot ID O08537
Protein Name estrogen receptor beta
Description of Target Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1 (ER-alpha), and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. May play a role in ovarian follicular gr
Kit Principle Genway Biotech Esr2 ELISA Kit (Mouse) (GWB-KBBXP1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Esr2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Esr2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Esr2 captured in the well.
Kit Component
ComponentAmount
Anti-XPC Microplate96 Wells (12 x 8 Well Strips)
XPC Lyophilized Standard2 x 20 ng
100X Biotinylated XPC Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_034287
Nucleotide Accession Num NM_010157
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXP1
Species Reactivity Mouse