EVL ELISA Kit (Human) (GWB-KBBOL5)

This exact product is now provided by Aviva Systems Biology.
Please visit OKDD00257 to review further and for ordering information.


GWB-KBBOL5

96 Wells
$849.00

Additional Information

Name EVL ELISA Kit (Human) (GWB-KBBOL5)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol EVL
Gene id 51466
Alias Symbols Ena/vasodilator-stimulated phosphoprotein-like, Ena/VASP-like protein, RNB6
Swissprot ID Q9UI08
Protein Name Ena/VASP-like protein
Description of Target Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and p
Kit Principle Genway Biotech EVL ELISA Kit (Human) (GWB-KBBOL5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EVL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EVL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EVL captured in the well.
Kit Component
ComponentAmount
Anti-EVL Microplate96 Wells (12 x 8 Well Strips)
EVL Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X EVL Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_057421.1
Nucleotide Accession Num NM_016337.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBOL5
Species Reactivity Human